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US 2019/0055537 A1Published application
Purification of cystathionine beta-synthase
AssigneeThe Regents of the University of Colorado, a body corporate
InventorsRichard Carrillo, Jan P. Kraus, Tomas Majtan, David Naveh
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Abstract
This invention provides chromatographic methods for the purification of a cystathionine β-Synthase (CBS) protein, particularly truncated variants thereof and compositions and pharmaceutical compositions prepared therefrom.
Claims25
A composition comprising a purified cystathionine β-synthase (CBS) protein produced by a method comprising the steps of:
- (a) providing a CBS protein-containing solution, said solution comprising one or more impurities;
- (b) first performing chromatographic separation of the CBS protein-containing solution of (a) using an ion exchange chromatography column;
- and
- (c) second performing a chromatographic separation using a metal affinity chromatography (IMAC) resin, wherein the one or more impurities are removed from the CBS protein-containing solution of (a),
- wherein the CBS protein of the CBS protein-containing solution of (a) comprises a chemically cleaved or genetically engineered carboxyl-terminal truncated CBS protein derived from SEQ ID NO:
- 2.
3 more elements
Dependencies are inferred from the claim text.