Factor vii polypeptides that are modified and uses thereof
AssigneeCatalyst Biosciences, Inc.
InventorsEdwin L. Madison, Christopher Thanos
Life of this patent
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Abstract
Nucleic acids encoding modified factor VII polypeptides, vectors and cells containing the nucleic acids, uses of the nucleic acids, methods of making the encoded polypeptides, and methods of treatment are provided. The encoded modified FVII polypeptides include Factor VIIa and other forms of Factor VII. Among the encoded modified FVII polypeptides provided are those that have altered activities, typically altered procoagulant activity, including increased procoagulant activities.
Claims23
A modified factor VII (FVII) polypeptide, comprising a modification at a position corresponding to position 286 in a FVII polypeptide having the sequence of amino acids set forth in SEQ ID NO:3 or in a corresponding residue at an aligned locus in a FVII polypeptide, wherein:
- the modification at position 286 is an amino acid replacement with Arg (R);
- the modified FVII polypeptide, when activated, exhibits increased coagulant activity compared to an unmodified FVII polypeptide that does not have the modification at position 286;
- the unmodified FVII polypeptide comprises a sequence of amino acids set forth in any of SEQ ID NOS:
- 1-3, 18-74, 98, 158 and 343-353;
- the modified FVII polypeptide has only 1, 2, 3, 4, 5, 6 or 7 amino acid modifications compared to the unmodified polypeptide;
- and
- the modifications are selected from an amino acid replacement, insertion or deletion.
Dependencies are inferred from the claim text.