Cellobiohydrolase Variants and Polynucleotides Encoding Same
AssigneeNovozymes A/S
InventorsKim Borch, Kenneth Jensen, Kristian Krogh, Brett McBrayer, Peter Westh, Jeppe Kari, Johan Olsen, Trine Sorensen, Michael Windhal, Hui Xu
Life of this patent
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Abstract
The present invention relates to cellobiohydrolase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims18
1-21. (canceled)
An isolated polynucleotide encoding a cellobiohydrolase I variant, wherein the cellobiohydrolase I variant has cellobiohydrolase I activity and increased specific performance relative to a cellobiohydrolase I comprising the amino acid sequence of amino acids 1 to 497 of SEQ ID NO:
- 2, wherein the cellobiohydrolase I variant comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of amino acids 1 to 497 of SEQ ID NO:
- 2, and wherein the Asn at the position corresponding to position 197 of the amino acid sequence of amino acids 1 to 497 of SEQ ID NO:
- 2 is substituted with Ala in the cellobiohydrolase I variant, the Ala at the position corresponding to position 199 of the amino acid sequence of amino acids 1 to 497 of SEQ ID NO:
- 2 is deleted in the cellobiohydrolase I variant, and the Asn at the position corresponding to position 200 of the amino acid sequence of amino acids 1 to 497 of SEQ ID NO:
- 2 is substituted with Ala or Trp in the cellobiohydrolase I variant.
Dependencies are inferred from the claim text.