Polypeptides
AssigneeNovozymes A/S
InventorsKlaus Gori, Henrik Marcus Geertz-Hansen, Jesper Salomon, Thomas Holberg Blicher, Mary Ann Stringer, Nikolaj Spodsberg, Tianqi Sun, Morten Gjermansen
Life of this patent
Estimating the term…
Abstract
The present invention relates to new polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent composition comprising polypeptides, a laundering method and the use of polypeptides.
Claims22
A composition comprising a polypeptide having DNase activity, wherein the polypeptide comprises any of the motifs [G/Y/W/F/A/H]NI[R/Q/D/E/V] (SEQ ID NO 73), SDH[D/H/L]P (SEQ ID NO 74) or GGNI[R/Q] (SEQ ID NO 75), and at least on additional component, selected from,
- v. a polyol, or
- vi. an enzyme preferably selected from protease and lipases, or
- vii. a surfactant, preferably selected from anionic or nonionic surfactants or
- viii. one or more polymer.
A composition according to any of the proceeding claims wherein the polymer is selected from (carboxymethyl)cellulose (CMC), poly(vinyl alcohol) (PVA), poly(vinylpyrrolidone) (PVP), poly(ethyleneglycol) and poly(ethylene oxide) (PEG).
A composition according to any of the proceeding claims wherein the composition is a detergent composition, such as a laundry or dish wash composition.
Use of a polypeptide having DNase activity, wherein the polypeptide comprises any of the motifs [G/Y/W/F/A/H]NI[R/Q/D/E/V] (SEQ ID NO 73), SDH[D/H/L]P (SEQ ID NO 74) or GGNI[R|Q] (SEQ ID NO 75) for preventing, reducing or removing a biofilm from an item, wherein the item is a textile.
Use according to any of the preceding claims for maintaining or improving the whiteness of the item.
Use according to any of the preceding claims, wherein a malodour is reduced or removed from the item.
A polypeptide having DNase activity, wherein the polypeptide comprises one or more of the motifs [G/Y/W/F/A/H]NI[R/Q/D/E/V] (SEQ ID NO 73), SDH[D/H/L]P (SEQ ID NO 74) or GGNI[R|Q] (SEQ ID NO 75) and wherein the polypeptide is selected from the polypeptides:
- a) a polypeptide having at least 72% sequence identity to the polypeptide of SEQ ID NO:
- 6,
- b) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO:
- 7,
- c) a polypeptide having at least 84.5% sequence identity to the polypeptide of SEQ ID NO:
- 10,
- d) a polypeptide having at least 82% sequence identity to the polypeptide of SEQ ID NO:
- 27,
- e) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 30,
- f) a polypeptide having at least 60% sequence identity to the polypeptide of SEQ ID NO:
- 33,
- g) a polypeptide having at least 65% sequence identity to the polypeptide of SEQ ID NO:
- 36,
- h) a polypeptide having at least 91% sequence identity to the polypeptide of SEQ ID NO:
- 39,
- i) a polypeptide having at least 99% sequence identity to the polypeptide of SEQ ID NO:
- 42,
- j) a polypeptide having at least 68% sequence identity to the polypeptide of SEQ ID NO:
- 45,
- k) a polypeptide having at least 94% sequence identity to the polypeptide of SEQ ID NO:
- 48,
- l) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 51,
- m) a polypeptide having at least 73% sequence identity to the polypeptide of SEQ ID NO:
- 57,
- n) a polypeptide having at least 90% sequence identity to the polypeptide of SEQ ID NO:
- 60,
- o) a polypeptide having at least 84% sequence identity to the polypeptide of SEQ ID NO:
- 63,
- p) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO:
- 66,
- q) a polypeptide having at least 72% sequence identity to the polypeptide of SEQ ID NO:
- 69, and
- r) a polypeptide having at least 68% sequence identity to the polypeptide of SEQ ID NO:
- 72.
a method of producing a polypeptide, wherein the polypeptide is selected from the group consisting of polypeptides in shown in SEQ ID NO 3, SEQ ID NO 4, SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8, SEQ ID NO 9, SEQ ID NO 10, SEQ ID NO 27, SEQ ID NO 30, SEQ ID NO 33, SEQ ID NO 36, SEQ ID NO 39, SEQ ID NO 42, SEQ ID NO 45, SEQ ID NO 48, SEQ ID NO 51, SEQ ID NO 54, SEQ ID NO 57, SEQ ID NO 60, SEQ ID NO 63, SEQ ID NO 66, SEQ ID NO 69, SEQ ID NO 72 and polypeptide having at least 80% sequence identity hereto, wherein the polypeptide has DNase activity
- (a) cultivating the recombinant host cell under conditions conducive for production of the polypeptide;
- and
- (b) recovering the polypeptide.
Dependencies are inferred from the claim text.