Dna polymerase variant
AssigneesKyushu University, National University Corporation, Takara Bio Inc.
InventorsYoshizumi ISHINO, Sonoko ISHINO, Takeshi YAMAGAMI, Takashi UEMORI, Nariaki TAKATSU
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Abstract
The present invention relates to a fusion polypeptide containing, in a direction of from an N-terminal side to a C-terminal side, one or more peptides which bind to a PCNA, and a polypeptide having a DNA polymerase activity; a method for amplifying nucleic acids using the polypeptide; and a composition and a kit, containing the polypeptide. According to the present invention, it is made possible to amplify a long-strand DNA in a short time in amplifying nucleic acids in the presence of PCNA even with a Pol I-type DNA polymerase.
Claims16
A fusion polypeptide comprising, in a direction of from an N-terminal side to a C-terminal side,
- a) one or more peptides which bind to a PCNA, and
- b) a polypeptide having a DNA polymerase activity.
Dependencies are inferred from the claim text.