Retrovirus Packaging Cell
AssigneeUCL Business PLC
InventorsMartin Pulé, Leila Mekkaoui, Gordon Weng-Kit Cheung
Life of this patent
Estimating the term…
Abstract
The present invention relates to a retrovirus packaging cell which expresses a temperature sensitive RNA-dependent-RNA polymerase (RdRp).
Claims37
A retrovirus packaging cell which expresses a temperature sensitive RNA-dependent-RNA polymerase (RdRp).
A retrovirus packaging cell according to any preceding claim wherein the RdRp is a Sindbis virus RdRp.
A retrovirus producer cell which is a packaging cell according to any preceding claim further comprising a retroviral nucleic acid transfer vector which comprises at least one RdRp promoter element which enables the retroviral nucleic acid transfer vector to be replicated by the RdRp.
A retrovirus packaging cell according to any of claims 1 to 5 or a producer cell according to any of claims 7 to 9 which comprises at least one retroviral helper element nucleotide sequence comprising at least one RdRp promoter element which enables a nucleic acid sequence which encodes a retroviral protein to be replicated by the RdRp.
A retrovirus packaging cell or producer cell according to any preceding claim which is a lentiviral packaging cell or a producer cell.
A packaging cell or producer cell according to any preceding claim which is a HEK293, HEK293-T, TE671, HT1080, 3T3, or K562 cell.
A retroviral nucleic acid transfer vector which comprises at least one RdRp promoter element which enables the retroviral nucleic acid transfer vector to be replicated by the RdRp.
A retroviral nucleic acid transfer vector comprising the following structure:
- 5′P1-rPSG-5dLTR-P2-NOI-3dLTR-PSG3′
- in which
- P1 is a eukaryotic promoter which drives transcription
- rPSG is a sequence which acts as a negative sense sub-genomic promoter for the RdRp when at the 3′ end of the negative sense strand;
- 5dLTR is a 5′ retrovirus long terminal repeat where the U3 region is non-functional;
- P2 is a eukaryotic promoter which drives expression of the NOI;
- NOI is a nucleotide sequence of interest;
- 3dLTR is a self-inactivating 3′ retrovirus long terminal repeat with a non-functional U3;
- PSG is a sequence which acts as a sub-genomic promoter for RdRp as a positive sense.
A retroviral helper element nucleotide sequence comprising at least one RdRp promoter element which enables a nucleic acid sequence which encodes a retroviral protein to be replicated by the RdRp.
Dependencies are inferred from the claim text.