Increased alcohol tolerance using the pntab gene
AssigneesThe United States of America as represented by the Secretary of Agriculture, The Regents of the University of California
InventorsSIQING LIU, CHRISTOPHER D. SKORY, DAVID A. MILLS
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Abstract
The pntAB locus may provide for increased alcohol tolerance in a microorganism, through expression of one or both of the pntA and pntB genes. A microorganism may have increased alcohol tolerance due to a transformation of the microorganism or an ancestor of the microorganism utilizing one or both of the pntA and pntB genes. The microorganism may be, for example, of a bacterial or fungal species. According to some exemplary embodiments, the microorganism may be a lactic acid bacterium.
Claims22
A transformed microorganism,
- wherein the transformed microorganism contains an alcohol tolerance gene,
- wherein the transformed microorganism has a higher alcohol tolerance as compared to a non-transformed microorganism, and
- wherein the alcohol tolerance gene is one of pntAB, pntA, and pntB, and
- wherein the microorganism expresses at least one alcohol tolerance protein, the alcohol tolerance protein being at least one of PntA and PntB.
(canceled)
A method of making a transformed microorganism having high alcohol tolerance, comprising:
- transforming a parental microorganism with a vector containing a DNA sequence encoding an alcohol tolerance gene, the alcohol tolerance gene being one of pntAB, pntA, and pntB;
- and
- thereby obtaining a transformed microorganism containing the alcohol tolerance gene,
- wherein the transformed microorganism has a higher alcohol tolerance than the parental microorganism, and
- wherein the transformed microorganism expresses at least one of the alcohol tolerance proteins PntA and PntB.
An isolated DNA sequence consisting essentially of a nucleotide sequence having at least 95% identity to one of SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 21, and SEQ ID NO:
- 22.
An expression vector comprising a promoter and a heterologous polynucleotide,
- wherein said heterologous polynucleotide encodes one or more proteins, the one or more proteins being at least one of PntA and PntB, and
- wherein said promoter is operatively linked to said heterologous polynucleotide.
Dependencies are inferred from the claim text.