Cell culture medium
OwnerSay Kong NG, Sow Zong Dawn LEONG +2inventors
InventorsSay Kong NG, Sow Zong Dawn LEONG, Gek Ling Janice TAN, Kah Hul Brian TEO
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Abstract
The invention relates to serum-free cell culture medium, wherein the medium either contains maltose as the sole carbohydrate source, or contains maltose and at least one other saccharide as carbohydrate sources. In a preferred embodiment, the additional saccharide is a monosaccharide, preferably glucose, and the medium comprises DMEM-F12. In a preferred embodiment the cells to be cultured may be CHO or HEK293 cells. Also disclosed are methods of growing and/or culturing a cell using the cell culture medium as described herein, methods of increasing protein yield using the cell culture medium as described herein, and kits thereof.
Claims27
A serum-free cell culture medium comprising maltose as sole carbohydrate source or a serum-free cell culture medium comprising maltose and at least one additional saccharide as carbohydrate sources.
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A method of growing and/or culturing a cell or a method of increasing protein yield, wherein the method comprises growing and/or culturing a cell in a serum-free cell culture medium comprising maltose as sole carbohydrate source or a serum-free cell culture medium comprising maltose and at least one additional saccharide as carbohydrate sources.
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A method of modulating glycosylation profile of a protein, wherein the method comprises culturing a cell expressing the protein in a serum-free cell culture medium to thereby produce a cell that expresses the protein with the modulated glycosylation profile, wherein the serum-free culture comprises maltose as sole carbohydrate source or a serum-free cell culture medium comprising maltose and at least one additional saccharide as carbohydrate sources.
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Dependencies are inferred from the claim text.