Methods and compositions for substituting membrane lipids in living cells
AssigneeThe Research Foundation for The State University of New York
InventorsErwin LONDON, Guangtao LI
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Abstract
The current disclosure provides methods, compositions and kits for substitution of cell membrane lipids in living cells. The current methods and compositions further provide methods for the efficient exchange of lipids with the endogenous lipids present in the outer leaflet of the cellular membrane. The methods and compositions of the current disclosure facilitate the exchange of lipids within the cellular membrane with those present in cyclodextrin-lipid complexes, which enables the utilization and analysis of membrane lipid composition, as well as the effect of altering the membrane lipid composition in living cells.
Claims36
A method for substituting lipids in a cell membrane comprising:
- providing a, cell, wherein said cell comprises a cellular membrane with a lipid bilayer;
- forming a cyclodextrin-lipid complex comprising at least one lipid bound to a cyclodextrin;
- and
- incubating said cell and said cyclodextrin-lipid complex in solution such that at least one lipid is exchanged between said lipid bilayer of said cell and said cyclodextrin-lipid complex.
A composition for substituting membrane lipids comprising:
- at least one α-cyclodextrin;
- and
- at least one lipid, wherein said at least one lipid is bound to said at least one α-cyclodextrin.
A kit for substituting lipids in a cellular membrane comprising;
- at least one α-cyclodextrin;
- at least one lipid
- instructions for forming a cyclodextrin-lipid complex comprising said least one lipid bound to said α-cyclodextrin;
- and
- instructions describing a method for using said at least one cyclodextrin-lipid complex to exchange said at least one lipid between a lipid bilayer of said cell membrane and said cyclodextrin-lipid complex.
Dependencies are inferred from the claim text.