Pseudomonas aeruginosa pcrv binding single variable domain antibodies
AssigneeAblynx N.V.
InventorsEvelyn De Tavernier, Ann Union, Bruno Dombrecht, Guy Hermans, Erika Morizzo
Life of this patent
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Abstract
Polypeptides are provided that are capable of significantly inhibiting and/or neutralizing P. aeruginosa. The polypeptides comprise two or more immunoglobulin single variable domains that are directed against the PcrV protein of P. aeruginosa, wherein the “first” immunoglobulin single variable domain and the “second” immunoglobulin single variable domain have different paratopes.
Claims40
Polypeptide comprising or consisting of two or more immunoglobulin single variable domains that are directed against PcrV, wherein at least one “first” immunoglobulin single variable domain is directed against a first epitope on PcrV and at least one “second” immunoglobulin single variable domain is directed against a second epitope on PcrV different from the first epitope on PcrV.
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Polypeptide that specifically binds PcrV of Pseudomonas aeruginosa, comprising an immunoglobulin single variable domain that essentially consists of 4 framework regions and 3 complementarity determining regions (CDRs), in which:
- CDR1 is chosen from the group consisting of:
- a) the amino acid sequences of SEQ ID NOs:
- 20-37;
- b) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs:
- 20-37;
- c) amino acid sequences that have 3, 2, or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs:
- 20-37;
- and/or
- CDR2 is chosen from the group consisting of:
- d) the amino acid sequences of SEQ ID NOs:
- 38-56;
- e) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs:
- 38-56;
- f) amino acid sequences that have 3, 2, or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs:
- 38-56;
- and/or
- CDR3 is chosen from the group consisting of:
- g) the amino acid sequences of SEQ ID NOs:
- 57-75;
- h) amino acid sequences that have at least 80% amino acid identity with at least one of the amino acid sequences of SEQ ID NOs:
- 57-75;
- i) amino acid sequences that have 3, 2, or 1 amino acid difference with at least one of the amino acid sequences of SEQ ID NOs:
- 57-75.
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The polypeptide according to claim 5, that binds to full length PcrV (SEQ ID NO:
- 159) and that shows reduced binding (30-90%) or no binding to chimera 7 (SEQ ID NO:
- 205).
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Dependencies are inferred from the claim text.