Trophic hormone fusion protein, preparation method and application thereof
AssigneeSuzhou Alphamab Co., Ltd.
InventorsTing XU, Kangping GUO, Lihong YUN
Life of this patent
Estimating the term…
Abstract
Disclosed herein is a gonadotropin fusion protein or a thyroid stimulating hormone fusion protein, a method for preparing the same and use thereof. β-subunit of the gonadotropin or thyroid stimulating hormone is fused to an Fc fragment directly or indirectly through a linker, and α-subunit binds to the β-subunit via an affinity between the α-subunit and the β-subunit. The fusion protein has a prolonged half-life and less fluctuating activity.
Claims19
A fusion protein comprising a trophic hormone protein having an α-subunit, a β-subunit and an Fc fragment of an antibody, wherein the β-subunit of the trophic hormone protein is linked to the Fc fragment directly or indirectly via a linker, and the α-subunit of the trophic hormone protein binds to the β-subunit via intermolecular interactions between the α-subunit and the β-subunit, and wherein the α-subunit of the trophic hormone is not linked to an Fc fragment.
(canceled)
A mixture comprising
- a heterodimeric protein comprising a fusion protein that comprises a trophic hormone protein having an α-subunit and a β-subunit and an Fc fragment of an antibody, wherein the β-subunit of the trophic hormone protein is linked to the Fc fragment directly or indirectly via a linker, and the α-subunit of the trophic hormone protein binds to the β-subunit via intermolecular interactions between the α-subunit and the β-subunit, wherein the α-subunit of the trophic hormone is not linked to an Fc fragment, and wherein the Fc chain is associated with the Fc fragment in the fusion protein via chemical association;
- and
- a homodimeric protein comprising two fusion proteins, wherein each fusion protein comprises a trophic hormone protein having an α-subunit and a β-subunit and an Fc fragment of an antibody, wherein the β-subunit of the trophic hormone protein is linked to the Fc fragment directly or indirectly via a linker, and the α-subunit of the trophic hormone protein binds to the β-subunit via intermolecular interactions between the α-subunit and the β-subunit, wherein the a-subunit of the trophic hormone is not linked to an Fc fragment, and wherein the Fc fragments in the two fusion proteins are associated with each other via chemical association.
6-11. (canceled)
A method for treating an abnormal trophic hormone disorder, comprising the step of administering to a mammal in need thereof an effective amount of a fusion protein comprising a trophic hormone protein having an α-subunit, a β-subunit and an Fc fragment of an antibody, wherein the β-subunit of the trophic hormone protein is linked to the Fc fragment directly or indirectly via a linker, and the α-subunit of the trophic hormone protein binds to the β-subunit via intermolecular interactions between the α-subunit and the β-subunit, wherein the α-subunit of the trophic hormone is not covalently linked to an Fc fragment.
Dependencies are inferred from the claim text.