Enhanced immunoglobulin diversity
AssigneeTrianni, Inc.
InventorsMatthias Wabl, Bao Duong, Nigel Killeen
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Abstract
The invention provides compositions and methods for enhanced production of immunoglobulin diversity. Specifically, the invention provides compositions and methods for making accessible a B cell receptor repertoire that has not been culled by developmental tolerance mechanisms. The invention also provides transgenic animals, cells, and antibodies resulting from these compositions and methods.
Claims28
A genetically modified animal comprising an immunoglobulin heavy chain allele that can undergo productive VDJ rearrangement but is deficient in expression of a functional immunoglobulin heavy chain from the immunoglobulin heavy chain allele, wherein the deficiency in expression can be corrected to allow for production of a functional immunoglobulin heavy chain that has not been subjected to selection by tolerance mechanisms.
A genetically modified animal comprising an immunoglobulin light chain allele that can undergo productive VJ rearrangement but is deficient in expression of a functional immunoglobulin light chain from the immunoglobulin light chain allele, wherein the deficiency in expression can be corrected to allow for production of a functional immunoglobulin light chain that has not been subjected to selection by tolerance mechanisms.
A method for producing a genetically modified animal, comprising the step of modifying a first immunoglobulin allele in the animal such that in-frame VDJ or VJ rearrangement is disabled for allelic exclusion but preserved for expression in an inducible manner.
Dependencies are inferred from the claim text.