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US 2019/0048378 A1Published application
Hemicellulase enriched compositions for enhancing hydrolysis of biomass
AssigneeDanisco US Inc.
InventorsSCOTT D POWER, SUZANNE E. LANTZ, EDMUND A. LARENAS, ROBERT M. CALDWELL
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Abstract
Described are compositions and methods relating to cellulase/hemicellulase enzyme blends for improving the enzymatic hydrolysis of cellulosic and hemicellulosic materials, as commonly found in biomass.
Claims15
1-30. (canceled)
A method for hydrolyzing a mixture of cellulosic and hemicellulosic materials comprising contacting the mixture with a hemicellulase-enhanced whole cellulase composition comprising:
- (a) a whole cellulase composition from Trichoderma reesei comprising one or more cellobiohydrolases, one or more endoglucanases, and one or more β-glucosidases, and
- (b) a hemicellulase composition comprising:
- a xylanase consisting essentially of an amino acid sequence having at least 95% identity to SEQ ID NO:
- 2, a β-xylosidase consisting essentially of an amino acid sequence having at least 95% identity to SEQ ID NO:17, and an arabinofuranosidase consisting essentially of an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 4,
- wherein the whole cellulase composition is in the range of 50% to 70% of the total protein in the hemicellulase-enhanced whole cellulase composition, and wherein the hemicellulase-enhanced whole cellulase composition provides enhanced glucan conversion and enhanced xylan conversion of a biomass substrate compared to an equivalent enzyme blend composition lacking the hemicellulase composition.
3 more elements
Dependencies are inferred from the claim text.