Expression method of haemocoagulase acutus (halase) recombinant protein

An expression method of a Haemocoagulase Acutus (Halase) recombinant protein, sequentially comprising the steps of optimizing a halase gene, performing Polymerase Chain Reaction (PCR) amplification on an optimized gene, constructing an expression vector, transfecting recombinant plasmids, and expressing the recombinant protein and identifying, wherein a method for constructing the expression vector is as follows:

• cutting a recycled PCR product and a pMCX cloning vector by an enzyme and connecting, constructing Agkis-pMCX plasmids, transforming the Agkis-pMCX plasmids to competent cells of an escherichia coli for amplification, screening in an Amp-resistant manner for positive cloning and sequencing, and extracting recombinant plasmids with correct sequencing;
• a method for transfecting the recombinant plasmids is as follows:
• transfecting the recombinant plasmids to CHO cells, wherein the recombinant plasmids containing a protein gene full length are transinfected independently and the recombinant plasmids containing an A sub-gene sequence and a B sub-gene sequence are co-transfected to the CHO cells.