Method of producing fatty acids
AssigneeTotal Raffinage Chimie
InventorsZhiwei ZHU, Anastasia KRIVORUCHKO, Jens NIELSEN
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Abstract
The present invention relates to a method for producing fatty acids, in particular shot/medium-chain fatty acids (S/MCFA), by expression in a host cell of a recombinant fatty acid synthase (FAS) naturally devoid of a thioesterase (TE) domain comprising a heterologous thioesterase (TE). The invention further relates to polynucleotides comprising such recombinant FAS, polypeptides encoded thereby, and vectors comprising such polynucleotides, as well as recombinant host cells comprising the polynucleotides, polypeptides, or vectors.
Claims15
A recombinant prokaryotic or eukaryotic cell comprising, expressing, or capable of expressing a fungal fatty acid synthase (FAS) and a thioesterase (TE), optionally wherein said FAS comprises FAS1 and FAS2.
A recombinant polynucleic acid encoding a fungal fatty acid synthase (FAS) or a FAS1 or FAS2 subunit thereof, and a thioesterase (TE).
(canceled)
Dependencies are inferred from the claim text.