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US 2019/0040432 A1Published application
Glucosyltransferase enzymes for production of glucan polymers
AssigneeE. I. Du Pont de Nemours and Company
InventorsMARK S. PAYNE, YEFIM BRUN, HONGXIAN HE, THOMAS SCHOLZ
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Abstract
Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
Claims21
1-14. (canceled)
A method for producing insoluble poly alpha-1,3-glucan comprising:
- a) contacting at least water, sucrose, and an isolated glucosyltransferase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:4, whereby insoluble poly alpha-1,3-glucan having at least 90% alpha-1,3 glycosidic linkages is produced;
- and
- b) isolating the insoluble poly alpha-1,3-glucan produced in step (a).
Dependencies are inferred from the claim text.