Cell seeding plate, method for manufacturing the same, and cell sheet separating method

A method for manufacturing a cell seeding plate comprising:

• providing a substrate comprising a surface;
• performing a surface treatment to the surface to bond a plurality of amino groups to the surface;
• and
• providing a plurality of photolysis compounds that reacts with the plurality of amino groups to form a photolysis layer, thereby forming the cell seeding plate, wherein each of the plurality of photolysis compounds has a chemical structural formula of
• R1 and R3 each represents alkane group, R2 comprises alkane group or olefin group, R5, R6 and R7 each represents hydrogen group or alkane group, the photolysis layer comprises a plurality of photolysis groups, each of the plurality of photolysis groups has a chemical structural formula of

A cell sheet separating method comprising:

• providing a cell seeding plate comprising:
• a substrate comprising a surface;
• and
• a photolysis layer formed on the surface;
• wherein the photolysis layer comprises a plurality of photolysis groups, each of the plurality of photolysis groups has a chemical structural formula of
• R1 and R3 each represents alkane group, R2 comprises alkane group or olefin group, R5, R6 and R7 each represents hydrogen group or alkane group, each of the plurality of photolysis groups is bonded to the surface by the amide group;
• seeding cells on the cell seeding plate to form a cell layer on a surface of the photolysis layer away from the substrate;
• and
• exposing the cell seeding plate to ultraviolet radiation to cause the ester group of the photolysis layer to be broken, thereby separating the cell layer from the substrate to obtain the cell sheet.