Use of the reductive glycine pathway for generating formatotrophic and autotrophic microorganisms
AssigneeYeda Research and Development Co. Ltd.
InventorsArren Bar-Even, Ron Milo, Elad Noor, Oren Yishai
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Abstract
An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.
Claims18
An isolated microoranism that is genetically modifed to express at least one enzyme of the reductive glycine pathway, said microorganism being able to utilize formate as its sole carbon source, wherein the microorganism is capable of converting formate to glycine and serine and further being able of converting said glycine and said serine to pyruvate or glycerate.
An isolated microorganism that is genetically modifed to express enzymes of the reductive glycine pathway, wherein the microorganism is capable of converting formate to a metabolite of central metabolism via the formation of glycine and without the formation of serine, said metabolite being selected from the group consisting of acetyl CoA, oxaloacetate, glycerate 2-phosphate and glycerate 3-phosphate.
A method of generating a microorganism comprising expressing in the microorganism at least one enzyme of the reductive glycine pathway, such that the microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine.
Dependencies are inferred from the claim text.