Herbicide-detoxifying enzymes and uses thereof
AssigneesSpogen Biotech Inc., Nufarm Limited
InventorBrian Thompson
Life of this patent
Estimating the term…
Abstract
Herbicide-detoxifying enzymes, compositions containing one or more of the enzymes, and plant seeds treated with the enzymes are provided. The enzymes can be used in methods for detoxifying auxin herbicides or degrading auxin plant regulators, including in methods for decontaminating surface of an apparatus used in agriculture or pesticide manufacturing, methods for decontaminating water, soil, soilless media, or sludge, and methods for protecting a plant from an auxin herbicide, improving a plant's tolerance to an auxin herbicide, or removing an auxin herbicide from the surface of a plant.
Claims36
1-12. (canceled)
An isolated nucleic acid comprising:
- (A) a nucleic acid sequence having at least 70%, identity to SEQ ID NO:
- 243, wherein:
- codon 84 is replaced with a codon that codes for a non-aromatic amino acid;
- codon 245 is replaced with a codon that codes for a non-aromatic amino acid;
- or
- both codons 84 and 245 are replaced with codons that code for a non-aromatic amino acids;
- (B) a nucleic acid sequence having:
- at least 97% identity to SEQ ID NO:
- 244 or 247, wherein codon 84 codes for a non-aromatic amino acid;
- at least 97% identity to SEQ ID NO:
- 245 or 248, wherein codon 245 codes for a non-aromatic amino acid;
- or
- at least 97% identity to SEQ ID NO:
- 246 or 249, wherein both codon 84 and codon 245 code for non-aromatic amino acids;
- (C) a nucleic acid sequence having at least 70% identity to SEQ ID NO:
- 250, wherein:
- codon 83 is replaced with a codon that codes for a non-aromatic amino acid;
- codon 241 is replaced with a codon that codes for a non-aromatic amino acid;
- or both codons 83 and 241 are replaced with codons that code for a non-aromatic amino acid;
- or
- (D) a nucleic acid sequence having:
- at least 70% identity to SEQ ID NO:
- 294 or 295, wherein codon 83 codes for a non-aromatic amino acid;
- at least 70% identity to SEQ ID NO:
- 296 or 297, wherein codon 241 codes for a non-aromatic amino acid;
- or
- at least 70% identity to SEQ ID NO:
- 298 or 299, wherein both codons 83 and 241 code for non-aromatic amino acids.
14-27. (canceled)
A composition comprising a carrier and an enzyme comprising:
- (a) an amino acid sequence having at least 70% identity to any one of SEQ ID NOs. 21-39, 41-70, 73, 126-138, 140-178, 251-258, 262, 268, 269, 270, 271, 272, 276, 277, 278, 281, 284, and 300-305, wherein the amino acid sequence comprises a substitution of the methionine at the first position of the sequence with a valine;
- (b) an amino acid sequence having at least 70% identity to any one of SEQ ID NOs. 21-39, 41-70, 73, 126-132, 134, 136, 137, 140-143, 146-157, 159, 160, 162-178, 251-258, 262, 268, 269, 270, 271, 272, 276, 277, 278, 281, 284, and 300-305, wherein the amino acid sequence comprises a substitution of the methionine at the first position of the sequence with a leucine;
- (c) an amino acid sequence having at least 70% identity to SEQ ID NO:
- 40, 71, or 261, wherein the amino acid sequence comprises a substitution of the valine at the first position of the sequence with a leucine;
- (d) an amino acid sequence having at least 70% identity to SEQ ID NO:
- 139, wherein the amino acid sequence comprises a substitution of the leucine at the first position of the sequence with a methionine or valine;
- (e) an amino acid sequence having at least 70% identity to SEQ ID NO:
- 251, wherein:
- the phenylalanine residue at amino acid position 84 of SEQ ID NO:
- 251 is substituted with a non-aromatic amino acid;
- the tyrosine residue at amino acid position 245 of SEQ ID NO:
- 251 is substituted with a non-aromatic acid;
- or
- both the phenylalanine residue at amino acid position 84 of SEQ ID NO:
- 251 and the tyrosine residue at amino acid position 245 of SEQ ID NO:
- 251 are substituted with non-aromatic amino acids;
- (f) an amino acid sequence having at least 70% identity to SEQ ID NO:
- 258, wherein:
- the phenylalanine residue at amino acid at position 83 of SEQ ID NO:
- 258 is substituted with a non-aromatic amino acid;
- the phenylalanine residue at amino acid at position 241 of SEQ ID NO:
- 258 is substituted with a non-aromatic amino acid;
- or
- both the phenylalanine residue at amino acid at position 83 of SEQ ID NO:
- 258 and the phenylalanine residue at amino acid at position 241 of SEQ ID NO:
- 258 are substituted with non-aromatic amino acids;
- (g) SEQ ID NO:
- 251, 252, 253, 254, 255, 256, 257, 300, 301, 302, 303, 304, or 305;
- or
- (h) an amino acid sequence having at least 70% identity to SEQ ID NO:
- 276, wherein the alanine residue at position 198 of SEQ ID NO:
- 276 is replaced with a valine residue.
29-34. (canceled)
37-47. (canceled)
A composition comprising a carrier and an enzyme that comprises an amino acid sequence having at least 70% identity to any one of SEQ ID NOs. 21-71, 73, 126-178, 251-258, 261, 262, 268-272, 276-278, 281, 284, and 300-305.
49-95. (canceled)
A composition comprising:
- a carrier;
- a 2,4-D-alpha ketoglutarate dioxygenase enzyme comprising an amino acid sequence having at least 70% identity to any one of SEQ ID NOs. 276-278;
- and
- a hydroxylase enzyme comprising an amino acid sequence having at least 70% identity to SEQ ID NO:
- 281.
97-116. (canceled)
120-132. (canceled)
135-136. (canceled)
138-148. (canceled)
151-161. (canceled)
A method for detoxifying an auxin herbicide or degrading an auxin plant growth regulator, comprising contacting the auxin herbicide or the auxin plant growth regulator with an enzyme, the enzyme comprising:
- a salicylate monooxygenase;
- a hydroxyphenylacetate monooxygenase;
- a hydroxybenzoate monooxygenase;
- a monooxygenase comprising an amino acid sequence having at least 70% identity to any one of SEQ ID NOs. 21-71, 251-258, 261, 262, 268-272, and 300-305;
- a phenolic acid decarboxylase comprising an amino acid sequence having at least 70% identity to SEQ ID NO:
- 73;
- a chlorohydrolase that is capable of detoxifying the auxin herbicide or degrading the auxin plant growth regulator;
- or
- a combination of any thereof.
163-236. (canceled)
A method for protecting a plant from an auxin herbicide, for improving a plant's tolerance for an auxin herbicide, or for removing an auxin herbicide from a surface of a plant, wherein the method comprises applying an enzyme to a plant, a plant seed, plant growth medium, or an area surrounding a plant or a plant seed and wherein the enzyme comprises a monooxygenase, a phenolic acid decarboxylase, a chlorohydrolase, or a combination of any thereof.
238-305. (canceled)
307-309. (canceled)
(canceled)
313-361. (canceled)
363-369. (canceled)
Dependencies are inferred from the claim text.