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US 2019/0024075 A1Published application
Methods and compositions for target detection
AssigneeArc Bio, LLC
InventorsStephane B. GOURGUECHON, Meredith L. CARPENTER, Eric HARNESS
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Abstract
Provided herein are methods and compositions for the detection and identification of targets in a sample, using target-specific guide nucleic acids and nucleic acid-guided nuclease system proteins.
Claims121
A method of identifying a target in a sample comprising:
- a. contacting nucleic acid from a sample with a plurality of guide nucleic acid (gNA)-nucleic acid-guided nuclease system protein complexes, wherein the complexes are targeted to at least one target, and wherein the nucleic acid, the gNA-nucleic acid-guided nuclease system protein complexes, or both the nucleic acid and the gNA-nucleic acid-guided nuclease system protein complexes comprise a label;
- and
- b. identifying the target in the sample, wherein the identifying is achieved by detecting a specific signal from the label, wherein the presence of a specific signal indicates binding of the gNA-nucleic acid-guided nuclease system protein complex to the nucleic acid.
A collection of guide nucleic acids (gNAs) targeted to at least one target, wherein a plurality of the gNAs comprise a label.
A collection of guide nucleic acid (gNA)-nucleic acid-guided nuclease system protein complexes, wherein the gNAs are targeted to at least one target, and wherein a plurality of the complexes comprise a label.
Dependencies are inferred from the claim text.