Back to results
US 2019/0017937 A1Published application
Microscope and method for observing biological specimen in living state
AssigneeJapan Science and Technology Agency
InventorsTakeharu Nagai, Yoshiyuki Arai, Kazushi Suzuki
Life of this patent
Estimating the term…
Abstract
A biological specimen containing a chemiluminescence substance that generates chemiluminescence is observed in a living state under a microscope. The microscope includes a light source that outputs control light that changes the state of the chemiluminescence, a defining unit that defines the radiation pattern of the control light with which an observation surface of the biological specimen is irradiated, and a detector that detects the chemiluminescence from the biological specimen.
Claims15
A method for observing a biological specimen in a living state, wherein the method comprises:
- an adding step of adding a luminescence substrate to the biological specimen containing a self-luminous fused protein that contains a luminescence enzyme and a fluorescent protein and generates chemiluminescence in cooperation with the luminescence substrate, and a luminescence control portion that is coupled with the luminescence enzyme and controls the chemiluminescence in accordance with whether or not the biological specimen is illuminated with control light that changes a state of the chemiluminescence;
- an illumination step of defining a radiation pattern of the control light and illuminating the biological specimen with the control light having the defined radiation pattern;
- and
- a detection step of detecting the chemiluminescence from the biological specimen.
1 more element
Dependencies are inferred from the claim text.