Variants of CPF1 (CAS12a) With Altered PAM Specificity
AssigneeThe General Hospital Corporation
InventorsJ. Keith Joung, Benjamin Kleinstiver, Alexander Sousa
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Abstract
Engineered CRISPR from Prevotella and Francisella 1 (Cpf1) nucleases with improved targeting range and enhanced on-target activity, and their use in genomic engineering, epigenomic engineering, base editing, genome targeting, genome editing, and in vitro diagnostics.
Claims60
An isolated CRISPR from Prevotella and Francisella 1 (Cpf1) protein from Acidaminococcus sp. BV3L6 (AsCpf1), comprising a sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:2 with mutations at one or more of the following positions:
- E174, S170, K548, N551, T167, T539, N552, M604, and/or K607 of SEQ ID NO:2.
An isolated CRISPR from Prevotella and Francisella 1 (Cpf1) protein from Lachnospiraceae bacterium ND2006 (LbCpf1), comprising a sequence that is at least 80% identical to SEQ ID NO:11, with one or more of the following positions:
- T152, D156, G532, and/or K538 of SEQ ID NO:11.
An isolated CRISPR from Prevotella and Francisella 1 (Cpf1) protein from Francisella tularensis (FnCpf1), comprising a sequence that is at least 80% identical to SEQ ID NO:4, with mutations at one or more of the following positions:
- K180, E184, N607, K613, D616, N617, and/or K671 of SEQ ID NO:4.
Dependencies are inferred from the claim text.