Apparatus and method for absolute quantification of biomarkers for solid tumor diagnosis
AssigneeQuanticision Diagnostics Inc.
InventorJiandi Zhang
Life of this patent
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Abstract
Provided is a method for quantitative analysis of a sample. The method includes steps of (a) providing a singular marker representative of one or more features of the sample, the sample comprising a population of individual units of the marker; (b) measuring the marker with dot blot analysis, wherein the quantitation result is an absolute amount of the marker's population of individual units in the sample, normalized by the sample volume or by the sample weight; and (c) obtaining an objective determination of the one or more features of the sample based on the quantitation result of the marker. Also disclosed is a reference database and a method to use the reference database for diagnosing cancer in a patient.
Claims20
A method for quantitative analysis of a sample, comprising:
- (a) providing a singular marker representative of one or more features of the sample, the sample comprising a population of individual units of the marker;
- (b) measuring the marker with dot blot analysis, wherein the quantitation result is an absolute amount of the marker's population of individual units in the sample, normalized by the sample volume or by the sample weight;
- and
- (c) obtaining an objective determination of the one or more features of the sample based on the quantitation result of the marker.
A reference database for diagnosing cancer in a patient based on quantitative analysis of a marker in a biopsy sample from the patient, comprising a plurality of reference profiles, each of the plurality of the reference profile prepared by:
- (a) providing a biopsy sample from a cancer patient with a known diagnosis;
- (b) measuring said marker with quantitative dot blot (QDB) analysis for the biopsy sample, wherein the quantitation result is an absolute amount of said marker in the biopsy sample, normalized by the biopsy sample volume or by the biopsy sample weight;
- and
- (c) associating the quantitation result with the known diagnosis of the cancer patient thereby obtaining a reference profile.
Dependencies are inferred from the claim text.