Production of manool
AssigneeFirmenich SA
InventorsMichel SCHALK, Letizia ROCCI
Life of this patent
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Abstract
Provided herein are methods of producing (+)-manool comprising: contacting geranylgeranyl diphosphate with an copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to a polypeptide selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO:2; and contacting the CPP with a sclareol synthase enzyme to form (+)-manool.
Claims22
A method of producing (+)-manool comprising:
- a. contacting geranylgeranyl diphosphate with an copalyl diphosphate (CPP) synthase to form a (9S,10S)-copalyl diphosphate wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to SEQ ID NO:
- 1 or SEQ ID NO:2;
- and
- b. contacting the CPP with a sclareol synthase enzyme to form (+)-manool;
- and
- c. optionally isolating the (+)-manool.
2-5. (canceled)
7-10. (canceled)
14-19. (canceled)
A method for transforming a host cell or non-human organism with a nucleic acid encoding a polypeptide having a copalyl diphosphate synthase activity and a polypeptide having a sclareol synthase activity wherein the polypeptide having the copalyl diphosphate synthase activity comprises an amino acid sequence that has at least 90%, 95%, 98%, 99% or 100% sequence identity to SEQ ID NO:
- 1 or SEQ ID NO:2 and wherein the polypeptide having the sclareol synthase activity comprises an amino acid sequence that has at least 90%, 95%, 98%, 99% or 100% sequence identity to SEQ ID NO:4 or SEQ ID NO:5.
An expression vector comprising a nucleic acid encoding a CPP synthase, wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to SEQ ID NO:
- 1 or SEQ ID NO:2 and a nucleic acid encoding a sclareol synthase.
Dependencies are inferred from the claim text.