Antibody and antibody mimetic for visualization and ablation of endogenous proteins
OwnerDon B. Arnold, Richard W. Roberts +3inventors
InventorsDon B. Arnold, Richard W. Roberts, Garrett G. Gross, Rudy Mora, Jason Junge
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Abstract
Provided are compositions and methods for labeling an endogenous protein, in particular, in a live cell, or for ablating an endogenous or target protein. The compositions relate to a fusion protein having a binding moiety such as an antibody, an antigen binding fragment of an antibody or an antibody mimetic that recognizes the endogenous or target protein.
Claims25
1-34. (canceled)
A method for treating a disease characterized by expression of an endogenous protein in a cell in a subject in need thereof, comprising administering to the subject a polynucleotide comprising:
- (1) a nucleic acid sequence encoding a fusion polypeptide, wherein the fusion polypeptide comprises
- (a) a binding moiety for recognizing an endogenous protein in the cell, and
- (b) a transcription factor and
- (2) a transcription regulatory element operatively linked to the nucleic acid sequence,
- wherein the transcription regulatory element regulates the transcription of the binding moiety and the transcription factor regulates the activity of the transcription regulatory element.
37-45. (canceled)
Dependencies are inferred from the claim text.