Method for manufacturing a microbial detection device, microbial detection method, microbial detection kit, and microbial detection device

A microbial detection kit, comprising:

• a detection device, including:
• a substrate having a sampling zone and a reacting zone, wherein the reacting zone includes a fiber material and a reacting reagent which comprises at least one selected from a group consisting of 5-methylphenazinium methosulfate and diaphorase;
• and at least one selected from a group consisting of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride (INT), water-soluble tetrazolium salts (WSTs), and (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT);
• and
• an acidic solution for treating the fiber material and the hydroxyl groups on a surface of the reacting zone which contact with the fiber material.

A microbial detection device, comprising:

• a substrate having a sampling zone and a reacting zone, wherein the reacting zone includes:
• a fiber material;
• and
• a reacting reagent, wherein the reacting reagent comprises at least one selected from a group consisting of 5-methylphenazinium methosulfate and diaphorase;
• and at least one selected from a group consisting of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride (INT), water-soluble tetrazolium salts (WSTs), and (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT);
• wherein the fiber material and hydroxyl groups of a surface of the reacting zone which contact with the fiber material are treated with an acidic solution.