Production of Anthocyanin from Simple Sugars
OwnerMichael Naesby, Zina Zokouri +3inventors
InventorsMichael Naesby, Zina Zokouri, David Fischer, Michael Eichenberger, Anders Hansson
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Abstract
Methods for producing anthocyanin by expression in a microorganism are disclosed including culturing of the microorganism under anthocyanin producing conditions, wherein the microorganism has an operative metabolic pathway including at least one heterologous enzyme activity, the pathway producing anthocyanin from simple sugars or other simple carbon sources.
Claims34
A microorganism, comprising an operative metabolic pathway capable of producing an anthocyanin from a simple sugar, the operative metabolic pathway comprising:
- a 4-coumaric acid-CoA ligase (4CL);
- a chalcone synthase (CHS);
- a flavanone 3-hydroxylase (F3H);
- a dihydroflavonol-4-reductase (DFR);
- an anthocyanidin synthase (ANS);
- an anthocyanidin 3-O-glycosyltransferase (A3GT);
- a chalcone isomerase (CHI);
- and
- at least one of
- a) a tyrosine ammonia lyase (TAL);
- or
- b) a phenylalanine ammonia lyase (PAL) and a trans-cinnamate 4-monooxygenase (C4H),
- wherein at least one enzyme of the operative metabolic pathway is encoded by a gene heterologous to the microorganism.
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A microorganism, comprising an operative metabolic pathway capable of producing an anthocyanin from a simple sugar, the operative metabolic pathway comprising:
- a 4-coumaric acid-CoA ligase (4CL);
- a chalcone synthase (CHS);
- a flavanone 3-hydroxylase (F3H);
- a dihydroflavonol-4-reductase (DFR);
- an anthocyanidin synthase (ANS);
- an anthocyanidin 3-O-glycosyltransferase (A3GT);
- a chalcone isomerase (CHI);
- at least one of
- a) a tyrosine ammonia lyase (TAL);
- or
- b) a phenylalanine ammonia lyase (PAL) and a trans-cinnamate 4-monooxygenase (C4H);
- and
- an anthocyanin-5-O-glycosyl transferase (A5GT), an anthocyanin-3-O-aromatic acyl transferase (A3AAT), or an anthocyanin-3-O-malonyl acyl transferase (A3MAT),
- wherein at least one enzyme of the operative metabolic pathway is encoded by a gene heterologous to the microorganism.
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Dependencies are inferred from the claim text.