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US 2018/0371507 A1Published application
Production of Tetrahydrocannabinolic Acid in Yeast
AssigneeLibrede Inc.
InventorsJason L. Poulos, Anthony N. Farnia
Life of this patent
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Abstract
Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
Claims3
A method for making tetrahydrocannabinolic acid, the method comprising:
- transforming S. cerevisiae with a first nucleotide sequence expressing an acyl-activating enzyme and expressing a mutant prenyltransferase;
- transforming the S. cerevisiae with a second nucleotide sequence expressing olivetolic synthase and expressing olivetolic acid cyclase;
- and
- transforming the S. cerevisiae with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 3 expressing aromatic prenyltransferase and expressing tetrahydrocannabinolic acid synthase.
1 more element
A method for making tetrahydrocannabinolic acid, the method comprising:
- transforming S. cerevisiae with a first nucleotide sequence expressing an acyl-activating enzyme and expressing a mutant prenyltransferase;
- transforming the S. cerevisiae with a second nucleotide sequence expressing olivetolic synthase and expressing olivetolic acid cyclase;
- and
- transforming the S. cerevisiae with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 19 expressing aromatic prenyltransferase and expressing tetrahydrocannabinolic acid synthase.
1 more element
A method for making tetrahydrocannabinolic acid, the method comprising:
- transforming S. cerevisiae with a first nucleotide sequence expressing an acyl-activating enzyme;
- transforming the S. cerevisiae with a second nucleotide sequence expressing a mutant prenyltransferase;
- transforming the S. cerevisiae with a third nucleotide sequence comprising the nucleotide sequence of SEQ. ID. NO. 3 expressing olivetolic synthase;
- transforming the S. cerevisiae with a fourth nucleotide sequence expressing olivetolic acid cyclase;
- transforming the S. cerevisiae with a fifth nucleotide sequence expressing aromatic prenyltransferase;
- and
- transforming the S. cerevisiae with a sixth nucleotide comprising the nucleotide sequence of SEQ. ID. NO. 29 expressing THC synthase.
4 more elements
Dependencies are inferred from the claim text.