A genetically modified yeast cell and improved process for production of clot-specific streptokinase
AssigneeCouncil of Scientific and Industrial Research
InventorsGirish Sahni, Kishore Kumar Joshi
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Abstract
Disclosed herein is an expression system for the production and secretion of biologically active clot-specific streptokinase (CSSK) protein in methylotrophic yeast. Yeast-expressed CSSK protein displays improved plasminogen activation and fibrin selectivity. Further disclosed are methylotrophic yeast transformed with at least one copy of functional cDNA sequence encoding CSSK adjunct with modified signal sequence which results in secretion of mature and correctly processed CSSK.
Claims23
An expression cassette comprising a polynucleotide, said polynucleotide comprising a yeast methanol inducible promotor sequence, a modified alpha signal gene sequence, a nucleic acid sequence encoding clot specific streptokinase and a transcription terminator sequence, wherein the nucleic acid sequence encoding clot specific streptokinase has an 85% identity to the nucleotide sequence selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6 and SEQ ID NO:
- 8.
A polynucleotide having a nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 23, SEQ ID NO:
- 26 and SEQ ID NO:
- 28.
Dependencies are inferred from the claim text.