Droplet libraries
AssigneeRaindance Technologies, Inc.
InventorsDarren Roy Link, Brian Hutchison, Michael L. Samuels, Michael Weiner
Life of this patent
Estimating the term…
Abstract
The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.
Claims30
-14. (canceled)
A method for detecting target molecules in a fluid sample, the method comprising:
- forming, in a microfluidic device, a plurality of separated partitions of fluid from a fluid sample comprising target molecules, wherein each of the plurality of partitions of fluid comprises a subvolume of the fluid sample, a single bead comprising a reactive component for binding a target molecule to the bead, and a detection component for binding to a target molecule bound to a bead to thereby form an immunocomplex, wherein the detection component comprises a detectable label;
- and
- monitoring each of the plurality of partitions of fluid for detection of an event associated with contents of one or more of the plurality of partitions of fluid.
The method of claim 6, further comprising conducting a reaction in the at least one of the plurality of partitions of fluid, wherein the reaction results in release of the detectable label.
A method for detecting target molecules in a fluid sample, the method comprising:
- forming, in a microfluidic device, a plurality of separated partitions of fluid from a fluid sample comprising target molecules, wherein each of the plurality of partitions of fluid comprises a subvolume of the fluid sample, a single bead comprising a reactive component for binding a target molecule to the bead, and a detection component for binding to a target molecule bound to a bead, the detection component comprises a detectable label;
- conducting a reaction in at least one of the plurality of partitions of fluid, wherein the detectable label is released;
- monitoring the reaction and detecting a signal from the released detectable label;
- and
- determining the presence of target molecules based on the detection of signals.
Dependencies are inferred from the claim text.