Viable viruses with foreign tags
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsKay S. Faaberg, Allyn Spear, Matthew A. Kappes, Kelly M. Lager
Life of this patent
Estimating the term…
Abstract
Multiple DIVA vaccines effective against porcine reproductive and respiratory syndrome virus (PRRSV) are disclosed. The DIVA vaccines may be negative DIVAs or positive DIVAs. The DIVA vaccines may be produced by modifying the nsp2 region of a modified live virus vaccine. The modification may be one or more deletions only (negative DIVAs) or a deletion with an insertion (positive DIVAs). The insertion may be of an epitope tag, such as a V5, S-Tag, or FLAG tag. Produced DIVA vaccines may be stable through multiple passes and thus may be effective for production and vaccination of animals.
Claims21
An immunogenic composition, comprising an isolated polynucleotide sequence representing a modified live vaccine for porcine reproductive and respiratory syndrome virus having an nsp2 region,
- wherein the nsp2 region has a modification at one of amino acids 10-14,
- wherein the modification comprises a deletion, and
- wherein the immunogenic composition is capable of inducing an immune response in a recipient.
An immunogenic composition, comprising an isolated polynucleotide sequence representing a modified live vaccine for porcine reproductive and respiratory syndrome virus having an nsp2 region,
- wherein the nsp2 region has a modification at amino acid 724-728,
- wherein the modification comprises a deletion, and
- wherein the immunogenic composition is capable of inducing an immune response in a recipient.
An immunogenic composition, comprising an isolated polynucleotide sequence representing a modified live vaccine for porcine reproductive and respiratory syndrome virus having an nsp2 region,
- wherein the nsp2 region comprises a deletion at a deletion starting point, the deletion starting point being one of amino acids 10-14 or one of amino acids 724-728, the deletion comprising the removal of 21-89 amino acids, and
- wherein the nsp2 region optionally comprises an insertion of an epitope tag of 10-25 amino acids in length at the deletion starting point.
A method for reducing the incidence of porcine reproductive and respiratory syndrome virus (PRRSV) in swine, comprising administering to a swine an immunogenic composition in an amount effective to generate an immune response in said swine to said PRRSV,
- wherein the immunogenic composition comprises an isolated polynucleotide sequence representing a modified live vaccine for porcine reproductive and respiratory syndrome virus having an nsp2 region,
- wherein the nsp2 region has a modification at one of amino acids 10-14 or one of amino acids 724-728, and
- wherein the modification comprises a deletion.
Dependencies are inferred from the claim text.