Methods for treating, preventing and diagnosing porcine epidemic diarrhea virus infection
AssigneeUniversity of Saskatchewan
InventorsVolker GERDTS, Brenda ALLAN, Jan VAN DEN HURK, Yan ZHOU, Alexander ZAKHARTCHOUK, Suresh TIKOO, Andrew POTTER
Life of this patent
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Abstract
Immunogens, compositions and methods for treating, preventing and/or diagnosing PEDV infection in pigs are disclosed. The compositions and methods use inactivated or attenuated virus-containing vaccines, or subunit vaccines, including immunogens and mixtures of immunogens derived from PEDV isolates.
Claims26
A composition comprising an inactivated or attenuated Porcine Epidemic Diarrhea Virus (PEDV);
- a pharmaceutically acceptable vehicle;
- and an immunological adjuvant selected from (a) alum or (b) an adjuvant composition comprising a host defense peptide, an immunostimulatory sequence and a polyphosphazine.
A composition comprising:
- (a) at least one isolated immunogen comprising an epitope from a PEDV spike (S) protein, a PEDV orf3 protein, a PEDV envelope (E) protein, a PEDV membrane (M) protein, and/or a PEDV nucleocapsid (N) protein;
- (b) a pharmaceutically acceptable vehicle;
- and
- (c) an immunological adjuvant.
A method of making a PEDV composition comprising;
- (a) inactivating or attenuating a PEDV;
- and
- (b) combining the inactivated PEDV with a pharmaceutically acceptable vehicle;
- and an immunological adjuvant selected from (i) alum or (ii) an adjuvant composition comprising a host defense peptide, an immunostimulatory sequence and a polyphosphazine.
A method of making a PEDV composition comprising;
- (a) providing at least one isolated immunogen comprising an epitope from a PEDV spike (S) protein, a PEDV orf3 protein, a PEDV envelope (E) protein, a PEDV membrane (M) protein, and/or a PEDV nucleocapsid (N) protein;
- and
- (b) combining the immunogen with a pharmaceutically acceptable vehicle;
- and an immunological adjuvant
An isolated PEDV nucleocapsid immunogen comprising at least one PEDV epitope, wherein the immunogen comprises the sequence of amino acids of SEQ ID NOS:28, 29 and/or 30, or the corresponding sequence from a non-USA/Colorado/2013 PEDV isolate.
25-26. (canceled)
Dependencies are inferred from the claim text.