Production of viruses in avian eggs
AssigneesCommonwealth Scientific and Industrial Research Organisation, University of Georgia Research Foundation, Inc.
InventorsAndrew Bean, John William Lowenthal, Luis Fernando Malaver-Ortega, Ralph A. Tripp
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Abstract
The present invention relates to modified avian eggs which can be used to produce increased levels of virus. The present invention also relates to methods of producing viruses in avian eggs of the invention, as well as the use of the viruses obtained to prepare vaccine compositions.
Claims34
An avian egg comprising;
- 1) a genetic modification which reduces the expression of an antiviral gene in the egg when compared to an isogenic egg lacking the genetic modification, and/or
- 2) an exogenous compound which reduces the expression of an antiviral gene and/or reduces the level of antiviral protein activity in the egg when compared to an isogenic egg lacking the compound,
- wherein the egg is capable of producing more virus than the isogenic egg.
A method of replicating a virus, the method comprising;
- 1) obtaining an avian egg,
- 2) administering a compound which reduces the expression of an antiviral gene and/or reduces the level of antiviral protein activity in the egg when compared to an isogenic egg lacking the compound,
- 3) inoculating the egg with the virus, and
- 4) incubating the egg for a predetermined period of time to replicate the virus.
A transgenic avian comprising a genetic modification, wherein the genetic modification reduces expression of an antiviral gene in an egg produced by the avian compared to an egg produced by an isogenic avian lacking the genetic modification.
Dependencies are inferred from the claim text.