Fast and highly specific dna-based multiplex detection
AssigneeAit Austrian Institute of Technology GmbH
InventorIvan Barisic
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Abstract
The present invention provides a method for multiplex detection of target DNA. Specifically the invention provides a solid substrate comprising a plurality of DNA probes, wherein each probe contains at least two linear nucleotide chains (LNCs). The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the multiple target detection are also provided.
Claims23
A detection system comprising a solid substrate and a plurality of DNA probes, wherein each probe contains at least two linear nucleotide chains (LNCs) linked together by a complementary nucleotide region, and wherein each probe further comprises:
- a. a first LNC having a nucleotide region complementary to a second LNC, the first LNC being immobilized to said substrate;
- and
- b. a third LNC comprising a nucleotide region complementary to the second LNC and a nucleotide region complementary to a nucleotide sequence of interest, and optionally
- c. at least one further LNC comprising two oligonucleotide regions complementary to other LNCs.
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Dependencies are inferred from the claim text.