Novel sucrose unassimilating flocculent yeast

Saccharomyces cerevisiae strain NITE BP-1587 obtained by a breeding method comprising:

• subjecting a yeast strain deposited under accession number NBRC 10055 and a yeast strain deposited accession number NITE BP-1589 to spore isolation to acquire an isolated spore showing type a or type a as a mating type;
• mating the isolated spore of the yeast strain deposited under accession number NBRC 10055 and the isolated spore of yeast strain deposited under accession number NITE BP-1589 which have different mating types to create a mated strain;
• carrying out, as for the created mated strain, tetrad dissection to acquire an isolated spore;
• and
• screening the obtained isolated spore to select the Saccharomyces cerevisiae yeast strain and having a character of a sucrose unassimilating property and flocculation.

A method of breeding a Saccharomyces cerevisiae yeast strain comprising:

• subjecting a yeast strain deposited under accession number NBRC 10055 and a yeast strain deposited under accession number NITE BP-1589 to spore isolation to acquire an isolated spore showing type a or type a as a mating type;
• mating the isolated spore of the yeast strain deposited under accession number NBRC 10055 and the isolated spore of the yeast strain deposited under accession number NITE BP-1589 which have different mating types to create a mated strain;
• carrying out, as for the created mated strain, tetrad dissection to acquire an isolated spore;
• and
• screening the obtained isolated spore to select the Saccharomyces cerevisiae yeast strain and having characters of a sucrose unassimilating property and flocculation.