Back to results
US 2018/0327728 A1Published application
Enzyme variants with improved ester synthase properties
AssigneeReg Life Sciences, LLC
InventorsAndrew M. SHUMAKER, Bernardo M. da Costa, Kevin HOLDEN, Louis G. HOM, Tarah S. BARON, Noah HELMAN
Life of this patent
Estimating the term…
Abstract
The disclosure relates to enzyme variants with improved ester synthase properties for the production of fatty acid esters. Further contemplated are recombinant host cells that express such variants, cell cultures comprising the recombinant host cells and fatty acid ester compositions produced by such recombinant host cells.
Claims2
1-142. (canceled)
A variant ester synthase polypeptide comprising SEQ ID NO:
- 67, wherein said variant ester synthase polypeptide is genetically engineered to have at least one mutation at an amino acid position selected from the group consisting of amino acids 4, 5, 7, 15, 24, 30, 33, 39, 40, 41, 43, 44, 58, 73, 76, 77, 78, 80, 98, 99, 101, 102, 111, 122, 131, 146, 147, 149, 150, 155, 157, 162, 164, 166, 170, 171, 172, 173, 177, 179, 182, 184, 185, 186, 187, 188, 190, 192, 193, 195, 197, 201, 202, 203, 206, 207, 212, 216, 219, 234, 239, 242, 243, 244, 246, 255, 257, 258, 259, 260, 262, 263, 264, 265, 266, 267, 285, 288, 289, 293, 294, 301, 302, 303, 304, 306, 307, 309, 310, 311, 313, 314, 315, 316, 317, 319, 320, 323, 328, 334, 348, 349, 351, 352, 353, 356, 357, 360, 366, 375, 381, 393, 394, 395, 409, 411, 413, 420, 424, 442, 443, 447, 454, 455, 457, 458, 461, 466, 468, and 472, wherein said variant ester synthase polypeptide has improved fatty acid ester activity compared to a corresponding wild type polypeptide.
Dependencies are inferred from the claim text.