Genetically engineered foot and mouth disease virus and related proteins, polynucleotides, compositions, methods and systems

A genetically modified Foot and Mouth Disease Virus (FMDV) having a viral capsid comprising an engineered FMDV VP1 protein, the engineered FDMV VP1 protein comprising

• an FMDV VP1 protein having an FMDV VP1 N-terminus and an FMDV VP1 C-terminus,
• a protein tag having up to 100 amino acids, and
• an FMDV 2A protein having an FMDV 2A N-terminus and an FMDV 2A C-terminus,
• wherein
• the FMDV VP1 C-terminus is fused to the FMDV 2A N-terminus and
• the protein tag is inserted in the FMDV VP1 protein at an insertion position selected from
• any one of position −1 to position +6 relative to the FMDV 2A N-terminus, or from any one of position −1 to position −7 relative to the FMD VP1 C-terminus,
• and wherein the protein tag is presented on an external surface of the viral capsid.