# US 2018/0321118 A1 · Biological sample treatment apparatus > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2018/0321118 A1 - Document ID: US20180321118A1 - Status: Published application - Kind: A1 - Application number: 16/035,848 - Filed: 2018-07-16 - Published: 2018-11-08 - Earliest claimed: 2011-11-16 - Assignees: Leica Biosystems Melbourne Pty Ltd - Applicants: Leica Biosystems Melbourne Pty Ltd - Inventors: Mark Brian DOCKRILL; Samuel GASON - Web page: https://patentgrep.com/patent/US20180321118A1 ## CPC - G01N 1/31: Apparatus therefor - G01N 1/312: {for samples mounted on planar substrates} - G01N 35/00029: {provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence)} - G01N 2035/00079: {Evaporation covers for slides} - G01N 2035/0094: {optimisation; experiment design} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Apparatus for treating biological samples disposed on substrates, including: an input buffer for receiving one or more substrate holders each being adapted to support a plurality of the substrates; a treatment zone including a plurality of treatment stations each being adapted to receive one of the substrates; a reagent dispenser configured by a controller to dispense reagents to the substrates at the treatment stations; a substrate transport device configured by the controller to transport individual substrates between the substrate holders in the input buffer and the treatment stations. ## Claims ### Claim 1 An automated method for treating biological samples disposed on substrates, wherein the substrates are treated at treatment stations in a treatment zone, the method including the steps of: receiving one or more of substrate holders in an input buffer, each substrate holder being adapted to support a plurality of the substrates; using a substrate transport device, configured by a controller, to transport individual ones of the substrates between the substrate holders in the input buffer and the treatment stations; and using a reagent dispenser, configured by the controller, to dispense reagents to the substrates at the treatment stations. ### Claim 2 (depends on 1) The method according to claim 1, and further including the step of: using the substrate transport device, configured by the controller, to transport the individual ones of the substrates between the treatment stations and the substrate holders in an output buffer for receiving the one or more of the substrate holders. ### Claim 3 (depends on 1) The method according to claim 1, and further including the step of: using the substrate transport device, configured by the controller, to transport the individual ones of the substrates into and out of a substrate covering unit for applying a cover member to the biological sample on the substrate. ### Claim 4 (depends on 1) The method according to claim 1, and further including the steps of: reading, using a substrate identification reader, a substrate identifier, borne by each of the substrates, when the substrate is the input buffer; using the controller to access a database for storing a plurality of protocols, each protocol defining a sequence of treatment operations to be applied to one or more of the substrates; and using the substrate transport device, configured by the controller, to group individual substrates within the treatment zone to efficiently treat the biological samples disposed on substrates. ### Claim 5 (depends on 4) The method according to claim 4, and further including one or more of the following steps: a. using the substrate transport device, configured by the controller, to group the individual ones of the substrates to which a common protocol is to be applied; b. using the substrate transport device, configured by the controller, to group the individual ones of the substrates to which a common reagent is to be applied; c. using the substrate transport device, configured by the controller, to group the individual ones of the substrates that share a common treatment time; d. using the substrate transport device, configured by the controller, to transport the individual ones of the substrates so as to optimise throughput of the substrates during protocols and/or treatment operations; and e. using the controller to maximise a number of protocols or treatment operations that can be consecutively performed on one or more of the substrates. ### Claim 6 (depends on 5) The method according to claim 5, and further including the step of: using the controller to optimise the a number of reagent dispensing operations performed by the reagent dispenser to different substrates without changing reagents. ### Claim 7 (depends on 4) The method according to claim 4, wherein the substrate identification reader is supported by the substrate transport device. ### Claim 8 (depends on 1) The method according to claim 1, wherein the reagent dispenser is supported by the substrate transport device. ### Claim 9 (depends on 1) The method according to claim 1, and further including the step of: transferring reagent, of the reagents and using a reagent transfer system, from a plurality of reagent containers, housed below a work surface on which the treatment stations are disposed, to the reagent dispenser. ### Claim 10 (depends on 9) The method according to claim 9, and further including the step of: aspirating the reagent, using an aspiration probe of the reagent transfer system, from the reagent containers via a reagent transfer port of the reagent transfer system, wherein the reagent transfer port passes through the work surface. ### Claim 11 (depends on 10) The method according to claim 10, and further including the step of: selectively bringing, using a carousel of the reagent transfer system supporting the reagent containers, each reagent container into fluidic communication with the reagent transfer port. ### Claim 12 (depends on 11) The method according to claim 11, and further including the step of: loading and unloading the reagent containers from the carousel via a reagent container access port of the reagent transfer system. ### Claim 13 (depends on 1) The method according to claim 1, and further including the step of: accessing, by using the substrate transport device configured by the controller, the substrates from the one or more of the substrate holders disposed in a plurality of input compartments of the input buffer. ### Claim 14 (depends on 13) The method according to claim 13, and further including the step of: baking the substrates in the substrate holders disposed in one or more baking stations of one or more of the input compartments. ### Claim 15 (depends on 13) The method according to claim 13, wherein the input compartments are adapted to support the substrate holders in a substantially horizontal orientation. ### Claim 16 (depends on 2) The method according to claim 2, and further including the step of: accessing, by using the substrate transport device configured by the controller, the substrates from the one or more of the substrate holders disposed in a plurality of output compartments of the output buffer. ### Claim 17 (depends on 16) The method according to claim 16, and further including the step of: curing the substrates in the substrate holders disposed in one or more curing stations of one or more of the output compartments. ### Claim 18 (depends on 16) The method according to claim 16, wherein the output compartments are adapted to support the substrate holders in a substantially horizontal orientation. ### Claim 19 (depends on 1) The method according to claim 1, wherein at least one of the substrate holders, received in the input buffer, supports a plurality of the substrates from a common patient case, and wherein the method further includes the step of: using the controller to group the plurality of the substrates into at least the one of the substrate holders received in an output buffer according to that common patient case. ### Claim 20 (depends on 1) The method according to claim 1, wherein the one or more of the substrate holders of the input buffer are ones of first substrate holders, and wherein the method further includes the steps of: using the substrate transport device, configured by the controller, to individually transport one of the substrates from a first slot in one of the first substrate holders in the input buffer to one of the treatment stations; and using the substrate transport device, configured by the controller, to individually transport the one of the substrates from the one of the treatment stations to a second slot in one of a plurality of second substrate holders in an output buffer. ### Claim 21 (depends on 20) The method according to claim 20, wherein the substrate transport device includes a robotic arm including a scanner and a suction cup at an end of the robotic arm, and wherein the method further includes using the robotic arm, configured by the controller, to perform the following steps: reach into the input buffer, scan, via the scanner attached to an end of the robotic arm, a label attached to one of the first substrate holders in a first label slot corresponding to the one of the substrates in the first slot; grip, via the suction cup attached to the end of the robotic arm, the one of the substrates from the first slot of the one of the first substrate holders; release the one of the substrates into one of the treatment stations; grip, via the suction cup, the one of the substrates from the one of the treatment stations; and release the one of the substrates into the second slot in the one of the plurality of the second substrate holders in the output buffer. ### Claim 22 (depends on 1) A non-transitory, computer readable medium comprising a computer program code which when executed implements the method of claim 1.