Phagemid Vector

A particle according to any preceding claim, wherein the genome of the recombinant phagemid particle comprises an origin of replication for enabling replication of double-stranded vector inside a prokaryotic host, optionally a pUC ori.

A particle according to any preceding claim, wherein the genome of the recombinant phagemid particle comprises one or more DNA sequence, which favours targeted integration into a host genome.

A particle according to any preceding claim, wherein the at least one transgene expression cassette comprises a viral transgene expression cassette.

A particle according to any preceding claim, wherein the at least one transgene expression cassette comprises a mammalian viral transgene expression cassette.

A particle according to any preceding claim, wherein the at least one transgene expression cassette comprises a lentivirus transgene expression cassette.

A particle according to any preceding claim, wherein the at least one transgene expression cassette comprises an adeno-associated virus (AAV) transgene expression cassette.

A particle according to any preceding claim, wherein the transgene expression cassette comprises a nucleic acid encoding an agent, which has therapeutic or industrial utility in the target cell or tissue.

A particle according to any preceding claim, wherein the transgene expression cassette comprises one or more functional elements required for expression of the nucleic acid in the target cell selected from the groups consisting of:

• a promoter, nucleic acid for encoding a polyA tail attachable to the expressed agent, left and/or right Inverted Terminal Repeat sequences (ITRs).

A particle according to any preceding claim, wherein the recombinant phagemid particle comprises one or more capsid minor coat protein, optionally wherein the recombinant phagemid particle comprises a pIII capsid minor coat protein that is configured to display a cell-targeting ligand for enabling delivery of the particle to the target cell.

A particle according to any preceding claim, wherein the recombinant phagemid particle comprises one or more capsid major coat protein, optionally wherein the recombinant phagemid particle comprises at least one pVIII capsid major coat protein that is configured to display a foreign peptide thereon.

A particle according to any preceding claim, wherein the genome of the recombinant phagemid particle lacks at least 60%, 70%, or at least 80% of the bacteriophage genome from which it is derived.

A particle according to any preceding claim, wherein the genome of the recombinant phagemid particle lacks at least 90%, 95%, or at least 99% of the bacteriophage genome from which it is derived.

A particle according to any preceding claim, wherein the phagemid particle lacks bacteriophage structural genes in its genome required for the formation, packaging or extrusion of the particle from a prokaryotic host, preferably structural genes which encode the capsid proteins.

A system for producing a recombinant phagemid particle from a prokaryotic host, the system comprising:

• (i) a first vector configured to persist inside a prokaryotic host, and comprising at least one transgene expression cassette, and a packaging signal for enabling replication of the vector into single-stranded DNA;
• and
• (ii) a second vector comprising nucleic acid encoding structural proteins required for packaging the single-stranded DNA, resulting in the formation and extrusion of a recombinant phagemid particle from the prokaryotic host.

A recombinant vector comprising comprising rAAV, rep-cap and adenohelper genes.