Lanosterol 14-alpha demethylase (cyp51) nucleic acid molecules that control pathogens
AssigneeDow AgroSciences LLC
InventorsJavier A. Delgado, Justin M. Lira, Chaoxian Geng, Meghan L. Frey
Life of this patent
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Abstract
This disclosure concerns nucleic acid molecules and methods of use thereof for control of pathogens through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in pathogens. The disclosure also concerns methods for applying dsRNA through formulations and/or transgenic plants that express nucleic acid molecules useful for the control of pathogens, and the plant cells and plants obtained thereby.
Claims21
A double-stranded nucleic acid comprising a ribonucleic acid (RNA) molecule that is specifically hybridizable with a polynucleotide selected from the group consisting of:
- SEQ ID NO:1;
- a complement of SEQ ID NO:1;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:1;
- a native coding sequence of a phytopathogen from the genus Zymoseptoria comprising any of SEQ ID NO:1;
- a fragment of at least 15 contiguous nucleotides of a native coding sequence of a phytopathogen from the genus Zymoseptoria comprising any of SEQ ID NO:1;
- SEQ ID NO:3;
- a complement of SEQ ID NO:3;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:3;
- SEQ ID NO:4;
- a complement of SEQ ID NO:4;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:4;
- SEQ ID NO:5;
- a complement of SEQ ID NO:5;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:5;
- SEQ ID NO:6;
- a complement of SEQ ID NO:6;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:6;
- SEQ ID NO:7;
- a complement of SEQ ID NO:7;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:7;
- SEQ ID NO:8;
- a complement of SEQ ID NO:8;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:8;
- SEQ ID NO:9;
- a complement of SEQ ID NO:9;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:9;
- SEQ ID NO:10;
- a complement of SEQ ID NO:10;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:10;
- SEQ ID NO:11;
- a complement of SEQ ID NO:11;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:11;
- SEQ ID NO:12;
- a complement of SEQ ID NO:12;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:12;
- SEQ ID NO:13;
- a complement of SEQ ID NO:13;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:13;
- SEQ ID NO:14;
- a complement of SEQ ID NO:14;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:14;
- SEQ ID NO:15;
- a complement of SEQ ID NO:15;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:15;
- and,
- SEQ ID NO:16;
- a complement of SEQ ID NO:16;
- a fragment of at least 15 contiguous nucleotides of SEQ ID NO:16.
A method for controlling a phytopathogen from the genus Zymoseptoria, the method comprising:
- (a) providing a ribonucleic acid (RNA) molecule to a phytopathogen, wherein the RNA is specifically hybridizable with a polynucleotide selected from the group consisting of any of:
- SEQ ID NOs:1 or 3-16;
- the complement of any of SEQ ID NOs:1 or 3-16;
- a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:1 or 3-16;
- the complement of a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:1 or 3-16;
- a transcript of any of SEQ ID NOs:1 or 3-16;
- the complement of a transcript of any of SEQ ID NOs:1 or 3-16;
- a fragment of at least 15 contiguous nucleotides of a transcript of any of SEQ ID NOs:1 or 3-16;
- and the complement of a fragment of at least 15 contiguous nucleotides of a transcript of any of SEQ ID NOs:1 or 3-16
- (b) contacting the RNA molecule with the pathogen, wherein the RNA molecule is taken up by the pathogen;
- (c) inhibiting a biological function within the pathogen, wherein the RNA molecule down regulates expression of a CytB gene endogenous nucleotide sequence.
A method of controlling an infestation of a phytopathogen from the genus Zymoseptoria in a plant, the method comprising contacting the phytopathogen with a ribonucleic acid (RNA) molecule that is specifically hybridizable with a polynucleotide selected from the group consisting of:
- SEQ ID NOs:1, 3-16;
- the complement of any of SEQ ID NOs:1, 3-16;
- a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:1, 3-16;
- the complement of a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:1, 3-16;
- a transcript of SEQ ID NOs:1, 3-16;
- the complement of a transcript of SEQ ID NOs:1, 3-16;
- a fragment of at least 15 contiguous nucleotides of a transcript of SEQ ID NOs:1, 3-16;
- and
- the complement of a fragment of at least 15 contiguous nucleotides of a transcript of SEQ ID NO:1, 3-16.
Dependencies are inferred from the claim text.