Methods for analyzing a tissue sample
AssigneePurdue Research Foundation
InventorsZheng Ouyang, Yu Xia, Xiaoxiao Ma
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Abstract
The invention generally relates to methods for analyzing a tissue sample. In certain aspects, the invention provides methods that involve obtaining a tissue sample including an unsaturated compound, conducting a radical reaction on the tissue sample that targets a carbon-carbon double bond within the unsaturated compound to thereby produce a plurality of compound isomers, subjecting the plurality of compound isomers to mass spectrometry analysis to identify a location of the carbon-carbon double bond within the unsaturated compound, and quantifying the plurality of compound isomers in order to distinguish normal tissue from diseased tissue.
Claims21
A method for analyzing a tissue sample, the method comprising:
- obtaining a tissue sample comprising an unsaturated compound;
- conducting a radical reaction on the tissue sample that targets a carbon-carbon double bond within the unsaturated compound to thereby produce a plurality of compound isomers;
- subjecting the plurality of compound isomers to mass spectrometry analysis to identify a location of the carbon-carbon double bond within the unsaturated compound;
- and
- quantifying the plurality of compound isomers in order to distinguish normal tissue from diseased tissue.
A method for analyzing a tissue sample, the method comprising:
- contacting a sampling probe to a tissue comprising an unsaturated compound in a manner in which the unsaturated compound is retained on the sampling probe;
- inserting the sampling probe into a hollow body, wherein reagents for a radical reaction are present within the hollow body and the radical reaction targets a carbon-carbon double bond within the unsaturated compound;
- conducting the radical reaction within the hollow body to produce reaction products;
- emitting the reaction products from a distal tip of the hollow body;
- and
- analyzing the emitted reaction products in a mass spectrometer in order to identify a location of the carbon-carbon double bond within the unsaturated compound.
21-27. (canceled)
Dependencies are inferred from the claim text.