Methods and compositions for reducing the tobacco specific nitrosamine nnk in tobacco
AssigneeNorth Carolina State University
InventorsRalph E. Dewey, Ramsey S. Lewis
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Abstract
The present invention provides a tobacco plant, plant part, and/or plant cell comprising one or more heterologous nucleic acid molecules comprising a nucleotide sequence encoding a pseudooxynicotine degrading enzyme. Further provided are methods and compositions for producing tobacco plants and tobacco products having reduced pseudooxynicotine (PON) and/or 4-(methyl nitrosoamino)-1-(3-pyridyl)-1-butanone (NNK) content.
Claims47
A tobacco plant, plant part, and/or plant cell comprising one or more heterologous nucleic acid molecules comprising a nucleotide sequence encoding a pseudooxynicotine (PON) degrading enzyme.
A method of reducing pseudooxynicotine (PON) and/or 4-(methyl nitrosoamino)-1-(3-pyridyl)-1-butanone (NNK) in a tobacco plant, plant part and/or plant cell, comprising:
- introducing into a tobacco plant, plant part and/or plant cell one or more heterologous nucleic acid molecules comprising a nucleotide sequence encoding a PON degrading enzyme, thereby reducing the PON and/or NNK content in the transgenic tobacco plant and/or plant part.
A method of producing a plant, plant part and/or plant cell having reduced pseudooxynicotine (PON) and/or 4-(methyl nitrosoamino)-1-(3-pyridyl)-1-butanone (NNK) content, comprising:
- introducing into a tobacco plant, plant part and/or plant cell one or more heterologous nucleic acid molecules comprising a nucleotide sequence encoding a PON degrading enzyme, thereby producing a tobacco plant, plant part and/or plant cell having reduced PON and/or NNK content.
A method of producing a tobacco product having reduced having reduced pseudooxynicotine (PON) and/or 4-(methyl nitrosoamino)-1-(3-pyridyl)-1-butanone (NNK) content, comprising:
- introducing into a tobacco plant, plant part and/or plant cell one or more heterologous nucleic acid molecules comprising a nucleotide sequence encoding a PON degrading enzyme, thereby producing a transgenic tobacco plant, plant part and/or plant cell having reduced PON and/or NNK content;
- and producing a tobacco product from said transgenic tobacco plant, plant part and/or plant cell, wherein the tobacco product has reduced PON and/or NNK content.
Dependencies are inferred from the claim text.