Stabilizer and stabilization method

A stabilization method for 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione or a salt thereof, wherein a compound represented by the general formula [1] is coexisted with 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione or a salt thereof;

• wherein, p pieces of M1 each independently represent a hydrogen atom or an alkali metal atom, q pieces of R1 each independently represent a hydroxy group or a sulfonic acid group represented by the general formula [A], m represents 0 or 1, p represents an integer of 1 to 3, q represents an integer of 0 to 4, Y represents a nitrogen atom or a CH group (a methine group), and Z represents a group represented by the general formula [Z-1], a group represented by the general formula [Z-2], or a group represented by the general formula [Z-3],
• wherein, M2 represents a hydrogen atom or an alkali metal atom,
• wherein, r pieces of R2 each independently represent a hydroxy group or the sulfonic acid group represented by the general formula [A], R3 represents a hydrogen atom or a hydroxy group, R4 represents a hydrogen atom or the sulfonic acid group represented by the general formula [A], R5 represents a hydrogen atom, a phenylamino group represented by the formula [B], or a naphthylazo group represented by the general formula [C], n represents 0 or 1, and r represents an integer of 0 to 4,
• wherein, R12 to R14 each independently represent a hydrogen atom, a hydroxy group, or the sulfonic acid group represented by the general formula [A],
• wherein, R6 to R9 each independently represent a hydrogen atom, a hydroxy group, or the sulfonic acid group represented by the general formula [A],
• wherein, R10 represents a hydrogen atom or a carboxylic acid group represented by the general formula [D], s pieces of R11 each independently represent a hydroxy group or the sulfonic acid group represented by the general formula [A], and s represents an integer of 0 to 5,
• wherein, M3 represents a hydrogen atom or an alkali metal atom.

A composition comprising 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione or a salt thereof, and a compound represented by the general formula [1];

• wherein, p pieces of M1 each independently represent a hydrogen atom or an alkali metal atom, q pieces of R1 each independently represent a hydroxy group or a sulfonic acid group represented by the general formula [A], m represents 0 or 1, p represents an integer of 1 to 3, q represents an integer of 0 to 4, Y represents a nitrogen atom or a CH group (a methine group), and Z represents a group represented by the general formula [Z-1], a group represented by the general formula [Z-2], or a group represented by the general formula [Z-3],
• in the formula, M2 represents a hydrogen atom or an alkali metal atom,
• wherein, r pieces of R2 each independently represent a hydroxy group or the sulfonic acid group represented by the general formula [A], R3 represents a hydrogen atom or a hydroxy group, R4 represents a hydrogen atom or the sulfonic acid group represented by the general formula [A], R5 represents a hydrogen atom, a phenylamino group represented by the formula [B], or a naphthylazo group represented by the general formula [C], n represents 0 or 1, and r represents an integer of 0 to 4,
• wherein, R12 to R14 each independently represent a hydrogen atom, a hydroxy group, or the sulfonic acid group represented by the general formula [A],
• wherein, R6 to R9 each independently represent a hydrogen atom, a hydroxy group, or the sulfonic acid group represented by the general formula [A],
• wherein, R10 represents a hydrogen atom or a carboxylic acid group represented by the general formula [D], s pieces of R11 each independently represent a hydroxy group or the sulfonic acid group represented by the general formula [A], and s represents an integer of 0 to 5,
• wherein, M3 represents a hydrogen atom or an alkali metal atom.

A kit for measuring a luminescence, comprising 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione or a salt thereof, and a compound represented by the general formula [1];

• wherein, p pieces of M1 each independently represent a hydrogen atom or an alkali metal atom, q pieces of R1 each independently represent a hydroxy group or a sulfonic acid group represented by the general formula [A], m represents 0 or 1, p represents an integer of 1 to 3, q represents an integer of 0 to 4, Y represents a nitrogen atom or a CH group (a methine group), and Z represents a group represented by the general formula [Z-1], a group represented by the general formula [Z-2], or a group represented by the general formula [Z-3],
• wherein, M2 represents a hydrogen atom or an alkali metal atom,
• wherein, r pieces of R2 each independently represent a hydroxy group or the sulfonic acid group represented by the general formula [A], R3 represents a hydrogen atom or a hydroxy group, R4 represents a hydrogen atom or the sulfonic acid group represented by the general formula [A], R5 represents a hydrogen atom, a phenylamino group represented by the formula [B], or a naphthylazo group represented by the general formula [C], n represents 0 or 1, and r represents an integer of 0 to 4,
• wherein, R12 to R14 each independently represent a hydrogen atom, a hydroxy group, or the sulfonic acid group represented by the general formula [A],
• wherein, R6 to R9 each independently represent a hydrogen atom, a hydroxy group, or the sulfonic acid group represented by the general formula [A],
• wherein, R10 represents a hydrogen atom or a carboxylic acid group represented by the general formula [D], s pieces of R11 each independently represent a hydroxy group or the sulfonic acid group represented by the general formula [A], and s represents an integer of 0 to 5,
• wherein, M3 represents a hydrogen atom or an alkali metal atom.