Multiplexed Genome Editing
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Luhan Yang, Marc Guell
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Abstract
A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.
Claims43
A method of inactivating expression of one or more target nucleic acid sequences in a cell comprising:
- introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein;
- and
- maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and inactivates the one or more target nucleic acid sequences in the cell.
A method of modulating one or more target nucleic acid sequences in a cell comprising:
- introducing into the cell a nucleic acid sequence encoding an RNA complementary to all or a portion of a target nucleic acid sequence in the cell;
- introducing into the cell a nucleic acid sequence encoding an enzyme that interacts with the RNA and cleaves the target nucleic acid sequence in a site specific manner;
- and
- maintaining the cell under conditions in which the RNA binds to complementary target nucleic acid sequence forming a complex, and
- wherein the enzyme binds to a binding site on the complex and modulates the one or more target nucleic acid sequences.
An engineered cell comprising:
- one or more endogenous viral genes;
- and
- one or more exogenous nucleic acid sequences that comprise a portion that is complementary to all or a portion of one or more target nucleic acid sequences of the one or more endogenous viral genes;
- wherein each of the one or more endogenous viral genes of the cell are modulated.
An engineered cell comprising:
- a plurality of endogenous retroviral genes;
- and
- one or more exogenous nucleic acid sequences that comprise a portion that is complementary to all or a portion of one or more target nucleic acid sequences of the plurality of endogenous viral genes;
- wherein each of the plurality of endogenous viral genes of the cell are modulated.
Dependencies are inferred from the claim text.