# US 2018/0282782 A1 · Designing Customized Protein-Specific Buffer System > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2018/0282782 A1 - Document ID: US20180282782A1 - Status: Published application - Kind: A1 - Application number: 15/761,936 - Filed: 2016-09-22 - Published: 2018-10-04 - Earliest claimed: 2015-09-22 - Assignees: Delta Tm Technologies - Applicants: Delta Tm Technologies - Inventors: Shawn CLARK; Jeffrey SIGNOR - Web page: https://patentgrep.com/patent/US20180282782A1 ## CPC - C12Q 1/34: involving hydrolase - B01L 3/50851: {specially adapted for heating or cooling samples} - C12Q 1/533: involving isomerase - C12Y 306/05005: Dynamin GTPase (3.6.5.5) - C12Y 502/01014: Beta-carotene isomerase (5.2.1.14) - G01N 33/6803: {General methods of protein analysis not limited to specific proteins or families of proteins} - B01L 2200/16: Reagents, handling or storing thereof - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12Y: ENZYMES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present invention is related to the field of protein chemistry. In particular, mixed buffer compositions are formulated that allow an accurate identification of agent-induced changes in protein melting point temperatures. Such buffer compositions provide for methods that determine the specific effects of exogenous agents on protein stability, cryoprotective effects and/or protein quality control (e.g., synthesis and/or extraction purity validations). ## Claims ### Claim 1 A device, comprising: a) a solid substrate comprising a plurality of testing wells and a control well, wherein each of said plurality of testing wells comprises a first buffer and a second buffer, said control well comprises a sample buffer, and each of said plurality of testing wells and control well comprises a protein sample; b) a first testing well series of said plurality of testing wells, wherein each well of said first testing well series comprises a different ammonium sulphate concentration; c) a second testing well series of said plurality of testing wells, wherein each well of said second testing well series comprises a different amino acid; d) a third testing well series of said plurality of testing wells, wherein each well of said third testing well series comprises a different urea concentration; e) a fourth testing well series of said plurality of testing wells, wherein each well of said fourth testing well series comprises a different glycerol concentration; f) a fifth testing well series of said plurality of testing wells, wherein each well of said fifth testing well series comprises a different hydrogen ion concentration; g) a sixth testing well series of said plurality of testing wells, wherein each well of said sixth testing well series comprises a different third buffer; h) a seventh testing well series of said plurality of testing wells, wherein each well of said seventh testing well series comprises a different dimethyl sulfoxide concentration; i) an eighth testing well series of said plurality of testing wells, wherein each well of said eighth testing well series comprises a different Hoefmeister Series compound; and j) a ninth testing well series of said plurality of testing wells, wherein each well of said ninth testing well series comprises an different metal ion. ### Claim 2 (depends on 1) The device of claim 1, wherein said device is configured for compatibility with a thermocycler. ### Claim 3 (depends on 1) The device of claim 1, wherein said device is configured for compatibility with an optical reaction module configured with an optically readable storage medium device. ### Claim 4 (depends on 1) The device of claim 1, wherein a first buffer is selected from the group consisting of MES, citrate and cacodylate. ### Claim 5 (depends on 1) The device of claim 1, wherein said second buffer is selected from the group consisting of bis-tris propane, bicine and HEPES. ### Claim 6 A device, comprising: a) a solid substrate comprising a plurality of testing wells and a control well, wherein each of said plurality of testing wells comprises a first buffer and a second buffer, said control well comprises a sample buffer, and each of said testing wells and control well comprises a protein sample; b) a first testing well series of said plurality of testing wells, wherein each well of said first testing well series comprises a different ethylene glycol concentration; c) a second testing well series of said plurality of testing wells, wherein each well of said second testing well series comprises a different polyethylene glycol 3350 MME concentration; d) a third testing well series of said plurality of testing wells, wherein each well of said third testing well series comprises a different erythritol concentration; e) a fourth testing well series of said plurality of testing wells, wherein each well of said fourth testing well series comprises a different 2-methyl-2,4 pentanediol concentration; f) a fifth testing well series of said plurality of testing wells, wherein each well of said fifth testing well series comprises a different polyethylene glycol 6000 concentration; g) a sixth testing well series of said plurality of testing wells, wherein each well of said sixth testing well series comprises a different sodium malonate concentration; h) a seventh testing well series of said plurality of testing wells, wherein each well of said seventh testing well series comprises a different ethanol concentration; i) an eighth testing well series of said plurality of testing wells, wherein each well of said eighth testing well series comprises a different polyethylene glycol 10,000 concentration; j) a ninth testing well series of said plurality of testing wells, wherein each well of said ninth testing well series comprises an different glycerol concentration; k) a tenth testing well series of said plurality of testing wells, wherein each well of said tenth testing well series comprises a different xylitol concentration; l) an eleventh testing well series of said plurality of testing wells, wherein each well of said eleventh testing well series comprises a different sucrose concentration; m) a twelfth testing well series of said plurality of testing wells, wherein each well of said twelfth testing well series comprises a different trehalose dihydrate concentration; n) a thirteenth testing well series of said plurality of testing wells, wherein each well of said thirteenth testing well series comprises a different 1,6 hexanediol concentration; and n) a fourteenth testing well series of said plurality of testing wells, wherein each well of said fourteenth testing well series comprises a different 1,2 propanediol concentration. ### Claim 7 (depends on 6) The device of claim 6, wherein said device is configured for compatibility with a thermocycler. ### Claim 8 (depends on 6) The device of claim 6, wherein said device is configured for compatibility with an optical reaction module configured with an optically readable storage medium device. ### Claim 9 (depends on 6) The device of claim 6, wherein said first buffer is selected from the group consisting of MES, citrate and cacodylate. ### Claim 10 (depends on 6) The device of claim 6, wherein said second buffer is selected from the group consisting of bis-tris propane, bicine and HEPES. ### Claim 11 A device, comprising: a) a solid substrate comprising a plurality of testing wells and a control well, wherein each of said plurality of testing wells comprises a first buffer and a second buffer, said control well comprises a sample buffer, and each of said testing wells and control well comprises a protein sample; b) a first testing well series of said plurality of testing wells comprising a first hydrogen ion concentration, wherein each well of said first testing well series comprises a different sodium chloride concentration; c) a second testing well series of said plurality of testing wells comprising a second hydrogen ion concentration, wherein each well of said second testing well series comprises a different sodium chloride concentration; d) a third testing well series of said plurality of testing wells comprising a third hydrogen ion concentration, wherein each well of said third testing well series comprises a different sodium chloride concentration; e) a fourth testing well series of said plurality of testing wells comprising a fourth hydrogen ion concentration, wherein each well of said fourth testing well series comprises a different sodium chloride concentration; f) a fifth testing well series of said plurality of testing wells comprising a fifth hydrogen ion concentration, wherein each well of said fifth testing well series comprises a different sodium chloride concentration; g) a sixth testing well series of said plurality of testing wells comprising a sixth hydrogen ion concentration, wherein each well of said sixth testing well series comprises a different sodium chloride concentration; h) a seventh testing well series of said plurality of testing wells comprising a seventh hydrogen ion concentration, wherein each well of said seventh testing well series comprises a different sodium chloride concentration; i) an eighth testing well series of said plurality of testing wells comprising an eighth hydrogen ion concentration, wherein each well of said eighth testing well series comprises a different sodium chloride concentration; and j) a ninth testing well series of said plurality of testing wells comprising a ninth hydrogen ion concentration, wherein each well of said ninth testing well series comprises a different sodium chloride concentration. ### Claim 12 (depends on 11) The device of claim 11, wherein said device is configured for compatibility with a thermocycler. ### Claim 13 (depends on 11) The device of claim 11, wherein said device is configured for compatibility with an optical reaction module configured with an optically readable storage medium device. ### Claim 14 (depends on 11) The device of claim 11, wherein said first buffer is selected from the group consisting of MES, citrate and cacodylate. ### Claim 15 (depends on 11) The device of claim 11, wherein said second buffer is selected from the group consisting of bis-tris propane, bicine and HEPES. ### Claim at position 16 16-66. (canceled)