Microbial approach for the production of 5-hydroxytryptophan
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsYajun Yan, Yuheng Lin
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Abstract
5-hydroxytryptophan (5-HTP), a precursor of serotonin, is produced in a microbial host cell. A modified bacterial phenylalanine 4-hydroxylase (P4H) catalyzes the tryptophan 5-hydroxylation reaction. Optionally the host cell includes a cofactor regeneration mechanism, allowing continuous production of 5-HTP without supplementation of exogenous cofactors.
Claims25
A genetically engineered bacterial cell comprising a modified bacterial phenylalanine-4-hydroxylase (P4H) wherein the modified P4H catalyzes the 5-hydroxylation of tryptophan in the presence of a tetrahydromonapterin (MH4) cofactor, and in the absence of a tetrahydrobiopterin (BH4) cofactor or 6,7-dimethyl-5,6,7,8-tetrahydropterine hydrochloride.
10-13. (canceled)
Dependencies are inferred from the claim text.