Chimeric adeno-associated virus/ bocavirus parvovirus vector
AssigneesUniversity of Iowa Research Foundation, University of Kansas
InventorsZiying Yan, John F. Engelhardt, Jianming Qiu
Life of this patent
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Abstract
The invention provides an isolated chimeric virus comprising bocavirus capsid protein and a recombinant adeno-associated viral (AAV) genome, an isolated rBoV comprising human bocavirus capsid protein and a recombinant BoV genome, and uses therefor.
Claims30
1-6. (canceled)
A method to express a heterologous gene product in mammalian cells comprising providing isolated chimeric virus comprising bocavirus (BoV) capsid protein and a recombinant adeno-associated viral (AAV) genome comprising an expression cassette encoding the heterologous gene product;
- and infecting the cells with the virus in an amount effective to express the heterologous gene product.
10-11. (canceled)
A method to inhibit or treat a condition associated with aberrant expression of an endogenous gene product, comprising:
- contacting a mammal at risk of or having the condition, with an effective amount of an isolated chimeric virus comprising bocavirus capsid proteins and a rAAV genome, wherein the rAAV genome comprises a transgene encoding at least a portion of a therapeutic gene product, the expression of which in the mammal inhibits or treats at least one symptom of the condition.
(canceled)
16-19. (canceled)
(canceled)
(canceled)
A method to immunize a mammal, comprising:
- administering to a mammal an isolated chimeric virus comprising bocavirus capsid proteins and a rAAV genome encoding a prophylactic gene product in an amount effective to prevent or inhibit microbial infection or replication.
27-48. (canceled)
51-58. (canceled)
Dependencies are inferred from the claim text.