Gene Optimized Hantaan Virus M Segment DNA Vaccine For Hemorrhagic Fever With Renal Syndrome

A DNA vaccine for hemorrhagic fever with renal syndrome caused by Hantaan virus (HTNV);

• said DNA expressing codon-optimized envelope glycoprotein genes of HTNV set forth in SEQ ID NO. 1.

A vector comprising the sequence of SEQ ID NO. 1.

A synthetic DNA construct comprising the optimized HTNV genes as set forth in SEQ ID NO. 2.

A vaccine for HTNV, SEOV and DOBV comprising:

• SEQ ID NO:
• 1 in or on a carrier.

A bivalent vaccine for HTNV and PUUV comprising a synthetic nucleic acid of SEQ ID NO. 1 and optimized PUUV DNA vaccine.

An isolated nucleic acid sequence set forth in SEQ ID No. 2.

A recombinant DNA construct comprising a vector and the DNA fragment comprising the nucleic acid sequence set forth in SEQ ID NO:

• 2.

A vaccine against infection of hantaviruses, said vaccine comprising a composition comprising inert particles and a nucleic acid of SEQ ID NO:

• 1 coated onto said particles.

A method of inducing a protective immune response against HTNV infection in a mammal, comprising the step of administering a vaccine of SEQ ID NO:

• 1

A synthetic, codon optimized HTNV full length M gene open reading frame of SEQ ID NO:

• 1 that encodes amino acids from viral proteins.

The synthetic, codon optimized HTNV full length M gene open reading frame of claim 30 that elicits antibodies when delivered in combination with optimized PUUV DNA vaccine.