Method for preparing a canine model of atherosclerosis
AssigneeBeijing Sinogene Biotechnology Co. Ltd.
InventorsMin Zheng, Jianping Zhao, Jidong Mi
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Abstract
The present invention relates to a method for preparing a canine model of atherosclerosis, in particular, relates to a method for preparing an apolipoprotein E (APOE) gene knock-out disease canine model with the use of gene knock-out technology.
Claims15
A method for establishing an APOE gene knock-out canine model, comprising the following steps:
- (1) obtaining a fertilized ovum or an oocyte from APOE gene knock-out canine prepared by gene editing technology;
- and (2) transplanting the fertilized ovum or the oocyte into one of the fallopian tubes of a female canine, of which both fallopian tubes have been embryo flushed.
A canine APOE gene targeting vector, consisting of an sgRNA sequence and its complementary sequence directed to a target site sequence of an exon of the canine APOE gene, and a skeleton vector.
Ear fibroblast BGD-APOEKO-EFO of APOE gene knock-out beagle canine, which is deposited in China General Microbiological Culture Collection Center (CGMCC) on Mar. 1, 2017 with a CGMCC depository No. 13804.
A primer pair for detecting APOE gene knock-out canine, comprising a genomic sequence comprising a sequence fragment as shown by cctggaccagggaggct (SEQ ID NO:
- 7), wherein the primer pair is designed to direct to the sequence as shown by cctggaccagggaggct (SEQ ID NO:
- 7).
Dependencies are inferred from the claim text.