Micropropagation of black barhee date palm
AssigneePhoenix Agrotech, LLC
InventorsKrishna Tewari, Anthony Fortier
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Abstract
Disclosed herein are media, kits, systems and methods for achieving micropropagation of a Black Barhee date palm on a commercially relevant scale. Compositions and methods for each stage of micropropagation, including initiation, elongation, and rooting are disclosed in the present application. Also disclosed are conditions for harvesting and sterilizing explant tissue for micropropagation.
Claims26
A method for micropropagating a Black Barhee date palm, comprising:
- applying a first medium in vitro to a Black Barhee date palm explant tissue comprising meristematic cells, at least until a shoot is initiated, wherein the first medium comprises:
- (a) 6-(gamma-gamma-dimethylallylamino)-purine (2-ip), 6-Benzylaminopurine (BAP) and Naphthoxyacetic acid (NOA);
- or
- (b) 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-(gamma-gamma-dimethylallylamino)-purine (2-iP) and kinetic (KIN);
- or
- (c) 6-Benzylaminopurine (BAP), kinetic (KIN), and 1-Naphthaleneacetic acid (NAA);
- and
- applying a second medium in vitro to the initiated shoot, wherein the second medium comprises 6-Benzylaminopurine (BAP).
A method for micropropagating a Black Barhee date palm, comprising:
- applying a first medium in vitro to a Black Barhee date palm explant tissue comprising meristematic cells, until a shoot is initiated, wherein the first medium comprises:
- (a) 6-(gamma-gamma-dimethylallylamino)-purine (2-ip), 6-Benzylaminopurine (BAP) and Naphthoxyacetic acid (NOA);
- or
- (b) 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-(gamma-gamma-dimethylallylamino)-purine (2-iP) and kinetic (KIN);
- or
- (c) 6-Benzylaminopurine (BAP), kinetic (KIN), and 1-Naphthaleneacetic acid (NAA).
A method for micropropagating a Black Barhee date palm, comprising applying a medium in vitro to an initiated Black Barhee date palm shoot derived from an explant, wherein the medium comprises 6-Benzylaminopurine (BAP).
A medium comprising the constituents of PAE-1, PAE1-M, PAE-9, PAE-21, PAE-71, PAE-19 or PAE-7.
Dependencies are inferred from the claim text.